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High-yield isolation of extracellular vesicles using aqueous two-phase system
Hyunwoo Shin1, Chungmin Han1, Joseph M Labuz2
1Department of Mechanical Engineering, POSTECH, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk, 790-784, Republic of Korea.
Scientific Reports
|August 15, 2015
Summary
Researchers developed an efficient extracellular vesicle (EV) isolation method using an aqueous two-phase system (ATPS). This rapid purification significantly enhances the diagnostic potential of EVs as blood-based biomarkers.
Area of Science:
- Biotechnology
- Biochemistry
- Medical Diagnostics
Background:
- Extracellular vesicles (EVs) are promising blood-based biomarkers.
- Effective purification is crucial for utilizing EVs in diagnostics.
- Current EV purification methods face limitations.
Purpose of the Study:
- To develop an efficient and rapid method for isolating extracellular vesicles (EVs).
- To improve the pre-analytical process for blood-borne EV diagnostic applications.
Main Methods:
- Utilized a polyethylene glycol/dextran aqueous two-phase system (ATPS) for EV isolation.
- Focused on achieving high EV recovery efficiency and short processing times.
Main Results:
- Achieved high EV recovery efficiency of approximately 70%.
- Completed the isolation process in a short timeframe of approximately 15 minutes.
- Demonstrated a method that significantly increases the diagnostic applicability of EVs.
Conclusions:
- The developed ATPS method offers an efficient and rapid approach for EV isolation.
- This purification strategy overcomes limitations of current methods.
- The technique enhances the potential of EVs as reliable diagnostic biomarkers.
