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Updated: Apr 5, 2026

Using Multi-fluorinated Bile Acids and In Vivo Magnetic Resonance Imaging to Measure Bile Acid Transport
Published on: November 27, 2016
Assay of Bile Acid Conjugation and Excretion in Human Hepatocytes
Helene Johansson1, Ewa C S Ellis
1Unit of Transplantation Surgery, Liver Cell Laboratory, Department of Clinical Investigation, Science and Technology (CLINTEC), Karolinska University Hospital Huddinge, Karolinska Institute, Stockholm, Sweden.
Abstract:
Primary hepatocytes isolated from human, mouse and rat liver as well as cell lines, such as HepG2 cells, are frequently used in vitro systems in liver research. In regenerative medicine, stem cells are used for differentiation towards hepatocyte-like cells with the goal of creating differentiated functional hepatocytes. It is therefore important to measure the quality and function of highly specialized hepatocyte-specific functions using appropriate methods. In this chapter, we describe an assay to assess conjugation and excretion of labeled bile acids in cultured hepatocytes.
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