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Draft Genome Sequence of Multidrug-Resistant Enterococcus faecium Clinical Isolate VRE3, with a Sequence Type 16

Saeed Khan1, Kidon Sung2, Bernard Marasa3

  • 1Division of Microbiology, National Center for Toxicological Research (NCTR), U.S. Food and Drug Administration, Jefferson, Arkansas, USA saeed.khan@fda.hhs.gov.

Genome Announcements
|August 15, 2015
PubMed

Insights

Multidrug-resistant Enterococcus faecium, a cause of hospital infections, was analyzed. A clinical strain (VRE3) with high vancomycin resistance was sequenced, revealing genetic elements linked to resistance.

Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Multidrug-resistant Enterococcus faecium is a significant nosocomial pathogen.
  • This bacterium can infect various body sites, including the gastrointestinal tract.
  • Infections pose serious implications for human health.

Purpose of the Study:

  • To present the draft genome sequence of a clinical strain of multidrug-resistant Enterococcus faecium.
  • To characterize the genetic basis of vancomycin resistance in the studied strain.

Main Methods:

  • Whole-genome sequencing of clinical strain VRE3.
  • Sequence type (ST) analysis.
  • Identification of mobile genetic elements associated with antibiotic resistance.

Main Results:

  • The clinical strain VRE3 was identified as sequence type 16 (ST16).
  • The genome contained a truncated Tn1546 element.
  • This element is associated with high-level vancomycin resistance.

Conclusions:

  • The genome sequence provides insights into the genetic makeup of a multidrug-resistant Enterococcus faecium strain.
  • Understanding the genetic elements like truncated Tn1546 is crucial for combating vancomycin resistance.
  • This data contributes to the study of hospital-acquired infections and antimicrobial resistance.

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