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An explanation of the noncorrespondence between assessment methods of cyclosporin
G M Randleff-Rasmussen1, M M Oosthuizen, J A Myburgh
1Department of Surgery, University of the Witwatersrand Medical School, Johannesburg, South Africa.
Abstract:
Various methods of determining cyclosporin (CyA) levels in patients after kidney transplantation were compared. These included polyclonal antibody (pcAb-), specific and nonspecific monoclonal antibody (S- and NmcAb-) radioimmunoassays (RIA), and high performance liquid chromatography (HPLC). The results obtained by the various methods when compared showed some correlation but did not correspond. A probable explanation for part of this noncorrespondence is the presence of monoclonally crossreactive metabolites (CyA-M). Another reason was that the concentration of CyA in the standards supplied with the RIA kits was found to be higher than stated.