Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Stress-driven design of intervertebral disc implants and optimization of mechanical and biological performance.

Journal of orthopaedic translation·2026

Same author

Mechanobiological research fueling the advancement of mechanotherapy and mechanohealth.

Mechanobiology in medicine·2026

Same author

Clinical and Radiological Spectrum in Cleidocranial Dysplasia: A Case Series.

Current health sciences journal·2025

Same author

Variability in Velar Morphology among Patients with Oral Submucous Fibrosis - A Cross-Sectional Survey in Odisha.

Journal of pharmacy & bioallied sciences·2025

Same author

Mechanobiology in cellular, molecular, and tissue adaptation.

Mechanobiology in medicine·2025

Same author

Ultrasound imaging and regulated mechanotransduction for characteristics, regeneration, and therapeutics of bone.

Mechanobiology in medicine·2025

Search research articles

Related Experiment Video

Updated: Apr 5, 2026

Mechanical Stimulation of Stem Cells Using Cyclic Uniaxial Strain

Mechanical Stimulation of Stem Cells Using Cyclic Uniaxial Strain

Published on: July 29, 2007

Dynamic Fluid Flow Mechanical Stimulation Modulates Bone Marrow Mesenchymal Stem Cells.

Minyi Hu¹, Robbin Yeh¹, Michelle Lien¹

¹Department of Biomedical Engineering, Stony Brook University, Stony Brook , NY 11794-5281, USA.

|August 15, 2015

Summary

Dynamic hydraulic stimulation (DHS) increases bone marrow mesenchymal stem cells (MSCs) in a time-dependent manner, peaking at 14 days. This mechanical loading enhances MSCs, potentially aiding osteoporosis treatment by promoting bone formation.

Keywords:

bone adaptation mechanical loading noninvasive stimulation osteopenia osteoporosis

More Related Videos

Purification, Expansion, and Flow Cytometry-Based Phenotyping of Mouse Derived Bone Marrow Mesenchymal Stem Cells

Purification, Expansion, and Flow Cytometry-Based Phenotyping of Mouse Derived Bone Marrow Mesenchymal Stem Cells

Published on: July 11, 2025

3D Magnetic Stem Cell Aggregation and Bioreactor Maturation for Cartilage Regeneration

3D Magnetic Stem Cell Aggregation and Bioreactor Maturation for Cartilage Regeneration

Published on: April 27, 2017

Related Experiment Videos

Last Updated: Apr 5, 2026

Mechanical Stimulation of Stem Cells Using Cyclic Uniaxial Strain

Mechanical Stimulation of Stem Cells Using Cyclic Uniaxial Strain

Published on: July 29, 2007

Purification, Expansion, and Flow Cytometry-Based Phenotyping of Mouse Derived Bone Marrow Mesenchymal Stem Cells

Purification, Expansion, and Flow Cytometry-Based Phenotyping of Mouse Derived Bone Marrow Mesenchymal Stem Cells

Published on: July 11, 2025

3D Magnetic Stem Cell Aggregation and Bioreactor Maturation for Cartilage Regeneration

3D Magnetic Stem Cell Aggregation and Bioreactor Maturation for Cartilage Regeneration

Published on: April 27, 2017

Area of Science:

Biomedical Engineering
Orthopedics
Stem Cell Biology

Background:

Osteoblasts, crucial for bone formation, originate from mesenchymal stem cells (MSCs).
Osteoporosis treatments aim to modulate MSC fate, as disuse hinders MSCs while mechanical signals promote osteogenesis.
Dynamic hydraulic stimulation (DHS) previously mitigated bone loss in a rat hindlimb suspension (HLS) model.

Purpose of the Study:

To investigate the downstream cellular effects of DHS on MSC populations in vivo.
To elucidate the mechanism by which DHS enhances bone quality under disuse conditions.
To determine the time-dependent response of bone marrow MSCs to DHS.

Main Methods:

A longitudinal in vivo study using Sprague Dawley rats subjected to hindlimb suspension (HLS) and/or DHS.
Bone marrow MSCs were isolated and quantified via flow cytometry at 3, 7, 14, and 21 days.
DHS was applied to the tibia using a specific loading regime (10 min on-5 min off-10 min on) daily for five days/week.

Main Results:

DHS induced a significant, time-dependent increase in bone marrow MSCs, with a peak effect observed on day 14.
The positive effect of DHS on MSC numbers diminished after 21 days.
MSC populations were positively influenced by mechanical signals from DHS.

Conclusions:

DHS mechanical loading positively influences the MSC pool, increasing their numbers.
DHS-induced changes in MSC quantity may promote osteoblastogenesis, aiding bone formation during disuse.
This study provides insights into time-sensitive MSC induction by mechanical loading for optimizing osteoporosis treatments.