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Updated: Apr 5, 2026

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DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis
Published on: October 6, 2017
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A poly(ADP-ribose) polymerase-1 activity assay based on the FRET between a cationic conjugated polymer and
Shiyun Tang1, Zhou Nie, Wang Li
1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China. niezhou.hnu@gmail.com wli@hnu.edu.cn.
Abstract:
A label-free and convenient strategy for PARP-1 activity assay and inhibitors assessment has been developed based on the fluorescence resonance energy transfer (FRET) between a cationic conjugated polymer (CCP) and supercharged green fluorescent protein (scGFP).
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