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Dialysis

Dialysis

Dialysis is a diffusion-based purification process that separates analyte molecules from a complex matrix. This is accomplished by allowing molecules in the solution to pass through a semipermeable membrane into a liquid on the other side. The membrane is usually made of cellulose acetate or cellulose nitrate, and the second liquid must be miscible with the solution. Ions (e.g., chloride or sodium) or organic molecules (e.g., glucose) can pass through the membrane pores, which generally have...

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Related Experiment Video

Updated: Apr 5, 2026

Custom-made Microdialysis Probe Design

Custom-made Microdialysis Probe Design

Published on: July 21, 2015

Custom-made Microdialysis Probe Design.

Jana Lietsche¹, Jan Gorka², Stefanie Hardt³

¹Department of Pharmacology, Goethe University of Frankfurt.

Journal of Visualized Experiments : Jove

|August 15, 2015

Summary

This summary is machine-generated.

Researchers developed a low-cost, custom microdialysis probe for neuroscience. This affordable tool enables monitoring of brain fluid changes, making advanced research accessible to more institutions.

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In Vivo Microdialysis Method to Collect Large Extracellular Proteins from Brain Interstitial Fluid with High-molecular Weight Cut-off Probes

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Published on: September 26, 2018

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Last Updated: Apr 5, 2026

Custom-made Microdialysis Probe Design

Custom-made Microdialysis Probe Design

Published on: July 21, 2015

Dissolved Solute Sampling Across an Oxic-Anoxic Soil-Water Interface Using Microdialysis Profilers

Dissolved Solute Sampling Across an Oxic-Anoxic Soil-Water Interface Using Microdialysis Profilers

Published on: March 24, 2023

In Vivo Microdialysis Method to Collect Large Extracellular Proteins from Brain Interstitial Fluid with High-molecular Weight Cut-off Probes

In Vivo Microdialysis Method to Collect Large Extracellular Proteins from Brain Interstitial Fluid with High-molecular Weight Cut-off Probes

Published on: September 26, 2018

Area of Science:

Neuroscience
Biomedical Engineering
Analytical Chemistry

Background:

Microdialysis is crucial for neuroscience research, enabling monitoring of physiological and pathological changes in cerebrospinal fluid.
Commercial microdialysis probes are costly, limiting accessibility for research groups in public institutions.

Purpose of the Study:

To detail the construction of a reliable, custom-made microdialysis probe for under $10.
To evaluate the in vitro recovery rates of the custom probe for substances of varying molecular weights and properties.

Main Methods:

Fabrication of a concentric microdialysis probe using a polysulfone membrane (30 kDa molecular cut-off).
In vitro recovery assessment of small compounds (glucose, lactate, acetylcholine, ATP) and neuropeptides.
Comparison of recovery rates based on molecular weight and physicochemical properties.

Main Results:

The custom probe achieved approximately 20% in vitro recovery for small compounds (100-500 Da).
In vitro recovery for neuropeptides (1,000-1,600 Da) ranged from 2-6%.
Dialysis of lower molecular weight compounds showed minimal impact on recovery rates.

Conclusions:

The custom-made microdialysis probe offers a cost-effective alternative to commercial probes.
The probe design demonstrates flexibility for accommodating different membrane materials and cut-off values.
This adaptable probe is suitable for microdialysis in rodent brain regions and can be modified for larger animals.