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PTCSC3 Is Involved in Papillary Thyroid Carcinoma Development by Modulating S100A4 Gene Expression
Jaroslaw Jendrzejewski1, Andrew Thomas1, Sandya Liyanarachchi1
1Human Cancer Genetics Program, Comprehensive Cancer Center (J.J., A.T., S.L., A.E., J.T., H.H., H.S.R., W.L., R.N., A.d.l.C.), The Ohio State University, Columbus, Ohio, 43210; and Department of Endocrinology and Internal Medicine (J.J., K.S.), Medical University of Gdansk, Poland.
The long noncoding RNA PTCSC3 suppresses S100A4, reducing papillary thyroid carcinoma (PTC) cell invasiveness. This suggests PTCSC3 plays a role in PTC development via the S100A4 pathway.
Area of Science:
- Molecular biology
- Genetics
- Cancer research
Background:
- A long noncoding RNA gene, PTCSC3, is located near the papillary thyroid carcinoma (PTC) risk variant rs944289.
- PTCSC3 may target the S100A4 gene, a potential factor in cancer development.
Purpose of the Study:
- To investigate the impact of PTCSC3 on S100A4 expression.
- To determine the role of PTCSC3 and S100A4 in thyroid cancer development.
Main Methods:
- Quantitative PCR (qPCR) to analyze S100A4 and PTCSC3 expression in tumor and unaffected thyroid tissue (n=73) and cell lines.
- Analysis of S100A4 target genes (VEGF, MMP-9) and cell motility/invasiveness assays in response to PTCSC3 expression.
Main Results:
- PTCSC3 significantly downregulated S100A4 expression in PTC cell lines.
- S100A4 was upregulated in tumor tissue, while PTCSC3 was downregulated.
- PTCSC3 expression suppressed S100A4, VEGF, and MMP-9, reducing PTC cell motility and invasiveness.
Conclusions:
- PTCSC3 downregulates S100A4, thereby reducing cell motility and invasiveness.
- PTCSC3 is proposed to influence PTC predisposition and carcinogenesis through the S100A4 pathway.
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