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Updated: Apr 5, 2026
Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients
Published on: June 16, 2017
Pilot study of DNA methylation in the pathogenesis of chronic rhinosinusitis with nasal polyps
Background:
DNA methylation has been implicated in the pathogenesis of allergy and atopy. This study aimed to identify whether DNA methylation also plays an important role in the pathogenesis of nasal polyps (NP).
Methodology:
NP tissues were obtained from 32 patients with chronic rhinosinusitis with bilateral NP. Biopsies of inferior turbinate mucosa (ITM) were taken from 18 patients who underwent rhinoseptoplasty (control group). The methylated genes, which were detected by DNA methylation microarray, were validated by methylation-specific polymerase chain reaction, bisulphite sequencing, real-time polymerase chain reaction and immunohistochemistry.
Results:
DNA methylation microarray identified 8,008 CpG islands in 2,848 genes. One hundred and ninety-eight genes were found to have a methylated signal in the promoter region in NP samples compared with ITM samples. The four top genes that changed, COL18A1, EP300, GNAS and SMURF1, were selected for further study. The methylation frequency of COL18A1 was significantly higher in NP samples than in ITM samples.
Conclusions:
DNA methylation might play an important role in the pathogenesis of NP. Promoter methylation of COL18A1 was found to be significantly increased in NP tissues, further studies are necessary to confirm the significance of these epigenetic factors in the mechanisms underlying the development or persistence of NP.
Insights
DNA methylation is linked to nasal polyps (NP) development. Promoter methylation of the COL18A1 gene was significantly increased in NP tissues, suggesting a role for these epigenetic changes in NP pathogenesis.
Area of Science:
- Epigenetics
- Molecular Biology
- Otolaryngology
Background:
- DNA methylation is implicated in allergic diseases.
- The role of DNA methylation in nasal polyp (NP) pathogenesis is unclear.
Purpose of the Study:
- To investigate the role of DNA methylation in the pathogenesis of nasal polyps (NP).
Main Methods:
- Compared DNA methylation profiles between NP tissues and inferior turbinate mucosa (ITM) from controls using DNA methylation microarray.
- Validated key gene methylation using methylation-specific PCR, bisulphite sequencing, real-time PCR, and immunohistochemistry.
Main Results:
- Identified 8,008 CpG islands across 2,848 genes with differential methylation.
- Found 198 genes with methylated signals in NP compared to ITM.
- Observed significantly higher promoter methylation frequency for COL18A1 in NP tissues.
Conclusions:
- DNA methylation may play a significant role in NP pathogenesis.
- Increased promoter methylation of COL18A1 in NP tissues warrants further investigation into its role in NP development and persistence.
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