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Updated: Apr 5, 2026
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Genome Editing in Mammalian Cell Lines using CRISPR-Cas
Published on: April 11, 2019
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CRISPR mediated somatic cell genome engineering in the chicken
Nadège Véron1, Zhengdong Qu1, Phoebe A S Kipen1
1EMBL Australia, Australian Regenerative Medicine Institute, Monash University, Building 75, Clayton, VIC 3800, Australia.
Developmental Biology
|August 17, 2015
Summary
Researchers used CRISPR gene editing in chicken embryos to create genetic mutations, enabling the study of gene function during development. This method efficiently targets genes like PAX7 for loss-of-function analysis in vivo.
Area of Science:
- Developmental biology
- Genetics
- Molecular biology
Background:
- Gene-targeted knockout technologies are essential for in vivo gene function studies.
- The CRISPR/Cas9 system offers revolutionary genome editing capabilities across various organisms.
Purpose of the Study:
- To establish an efficient method for gene targeting in chicken embryos using CRISPR.
- To investigate the function of the transcription factor PAX7 in embryonic development.
Main Methods:
- CRISPR/Cas9 system combined with in vivo electroporation.
- Targeting of the transcription factor PAX7 in developing chicken embryo tissues.
- Generation of mosaic genetic mutations within a wild-type cellular background.
Main Results:
- Successful in vivo genome engineering in chicken embryos.
- Efficient targeting of the PAX7 gene.
- Generation of mosaic mutations for loss-of-function studies.
Conclusions:
- In vivo CRISPR-mediated genome engineering is effective for gene loss-of-function studies in chicken embryos.
- This technique enhances the genetic toolbox for studying embryonic development in chickens.
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