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MicroRNA-141 regulates the tumour suppressor DLC1 in colorectal cancer

P P Wu, H Y Zhu, X F Sun

    Neoplasma
    |August 18, 2015
    PubMed

    Insights

    MicroRNA-141 (miR-141) promotes colorectal cancer (CRC) growth and invasion by targeting the tumor suppressor DLC1. This study reveals miR-141 as a potential oncogene in CRC, offering new therapeutic targets.

    Area of Science:

    • Oncology
    • Molecular Biology
    • Genetics

    Background:

    • Previous research identified DLC1 as a tumor suppressor in colorectal cancer (CRC).
    • The role of microRNA (miRNA) regulation in DLC1 function within CRC remained unclear.

    Purpose of the Study:

    • To investigate if DLC1 is a target of miRNA regulation in CRC.
    • To evaluate the functional role of the miR-141/DLC1 axis in colorectal cancer progression.

    Main Methods:

    • Bioinformatic analysis and literature review identified miR-141.
    • In vitro studies involved transfecting Lovo cells with miR-141 mimic/inhibitor.
    • Cell growth, cell cycle, invasion, and gene expression were assessed using MTT assays, flow cytometry, transwell assays, luciferase reporter assays, and Western blots.
    • In vivo tumor models and analysis of clinical CRC samples were conducted.

    Main Results:

    • DLC1 was confirmed as a direct target of miR-141 in CRC.
    • miR-141 was significantly upregulated, and DLC1 downregulated in CRC tissues, showing a negative correlation.
    • miR-141 overexpression enhanced CRC cell proliferation, cell cycle progression, and invasion in vitro and in vivo.
    • Restoring DLC1 expression in miR-141-overexpressing cells suppressed tumor growth and invasion.

    Conclusions:

    • miR-141 is upregulated in colorectal cancer and functions as an oncogene.
    • The oncogenic role of miR-141 in CRC is mediated through the suppression of its target, DLC1.
    • The miR-141/DLC1 pathway represents a potential therapeutic target for colorectal cancer treatment.
    Keywords:
    DLC1.colorectal cancermiR-141

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