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Visualization of Endoplasmic Reticulum Localized mRNAs in Mammalian Cells
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Visualization of Endoplasmic Reticulum Localized mRNAs in Mammalian Cells

Published on: December 17, 2012

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Enzyme function is regulated by its localization

Stacey M Gifford1, Pablo Meyer1

  • 1IBM T. J. Watson Research Center, Yorktown Heights, NY 10598, United States.

Computational Biology and Chemistry
|August 18, 2015
PubMed

Insights

Enzyme localization is crucial for function. The glycosyltransferase MurG

Area of Science:

  • Microbiology
  • Cell Biology
  • Biochemistry

Background:

  • Enzyme activity is influenced by cellular localization.
  • The glycosyltransferase MurG is essential for cell wall synthesis during Bacillus subtilis sporulation.

Purpose of the Study:

  • To investigate the role of MurG's cellular localization in its enzyme activity during Bacillus subtilis sporulation.
  • To determine the factors regulating MurG localization to the forespore membrane.

Main Methods:

  • Studied the cellular localization of MurG in Bacillus subtilis during sporulation.
  • Utilized point mutations in MurG to disrupt membrane localization.
  • Examined MurG localization in strains with deleted cardiolipin-synthesizing genes.
  • Assessed the effect of external cardiolipin addition on MurG localization.

Main Results:

  • MurG gradually enriched to the forespore membrane during sporulation.
  • Disrupting MurG's membrane localization via mutations caused sporulation defects.
  • MurG localization was dependent on the phospholipid cardiolipin.
  • Absence of cardiolipin diminished MurG at the forespore, which was rescued by cardiolipin addition.

Conclusions:

  • Cellular localization is a critical regulatory factor for enzyme function.
  • MurG's localization to the forespore membrane, dependent on cardiolipin, is essential for Bacillus subtilis sporulation.
Keywords:
CardiolipinCell wallEnzyme localizationMurG

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