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α-Klotho Expression in Human Tissues.

Kenneth Lim1, Arnoud Groen1, Guerman Molostvov1

  • 1Renal Division (K.L., T.L., L.-L.H.), Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115; Cambridge Centre for Proteomics (A.G., K.S.L., T.F.H.), and School of Clinical Medicine (I.B.W., T.F.H.), University of Cambridge, Cambridge CB2 0QQ, United Kingdom; Department of Pathology (D.S., S.J., S.T.), University Hospitals Coventry and Warwickshire, NHS Trust, Coventry CV2 2DX, United Kingdom; and Division of Translational Research (G.M., D.Z.), Warwick Medical School, University of Warwick, Coventry CV2 2DX, United Kingdom.

The Journal of Clinical Endocrinology and Metabolism
|August 18, 2015
PubMed
Summary
This summary is machine-generated.

This study maps the expression of alpha-Klotho (α-Klotho), a key aging regulator, across human tissues. Researchers confirmed its presence in kidney tubules and arteries, clarifying its distribution in human health.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Gerontology

Background:

  • Alpha-Klotho (α-Klotho) is recognized as a significant regulator of the aging process.
  • The precise expression profile of α-Klotho in human tissues remains largely uncharacterized and debated.
  • Understanding α-Klotho distribution is crucial for its role in aging and disease.

Purpose of the Study:

  • To systematically map the tissue expression and spatial distribution of transmembrane α-Klotho in humans.
  • To provide the first comprehensive characterization of human α-Klotho expression across various organ systems.
  • To utilize advanced proteomic and antibody-based techniques for accurate detection.

Main Methods:

  • Employed next-generation targeted proteomic analysis, specifically parallel reaction monitoring (PRM).
  • Utilized conventional antibody-based methods, including immunohistochemistry and Western blotting.
  • Validated findings using mass spectrometry to identify α-Klotho-specific peptides.

Main Results:

  • Identified α-Klotho expression in arterial, epithelial, endocrine, reproductive, and neuronal tissues.
  • Demonstrated strong α-Klotho expression in kidney tissues, with no detectable signal in the liver.
  • Confirmed results via Western blotting and PRM mass spectrometry, identifying the full-length transmembrane isoform.

Conclusions:

  • The study confirms α-Klotho expression in kidney tubules and arteries.
  • Provides evidence for α-Klotho expression across diverse human organ systems and cell types.
  • This detailed mapping advances our understanding of α-Klotho's role in human physiology and aging.