Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Continuous <i>in silico</i> screening of fish, amphibians, reptiles, and birds expands the diversity of bornavirids and reveals unexpected genomic architectures.

Virus evolution·2026
Same author

Emerging Goatpox Virus Threat in Wild Ruminants: First Documented Outbreak in the United Arab Emirates, 2024.

Veterinary sciences·2026
Same author

Aujeszky's Disease in a Grey Wolf (<i>Canis lupus</i>) in Poland.

Viruses·2026
Same author

A novel genetically distinct Amdoparvovirus in Sorex araneus in the United Kingdom highlights an unexplored ancestral link.

Virus research·2026
Same author

Persistent foot-and-mouth disease virus infection of the bovine nasopharynx is associated with suppression of innate and cellular immunity.

PloS one·2026
Same author

A metagenomic analysis of urban river samples reveals high numbers of sequences related to mycoviruses.

Archives of virology·2026

Related Experiment Video

Updated: Apr 5, 2026

Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining
13:22

Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining

Published on: January 23, 2014

18.8K

Novel method for genotyping clinical herpes simplex virus type 1 isolates.

Brigitte Glück1, Susanne Möbius1, Florian Pfaff1

  • 1Institute of Virology and Antiviral Therapy, German Consulting Laboratory for HSV and VZV, Jena University Hospital, Friedrich Schiller University Jena, Hans-Knöll-Str. 2, 07745, Jena, Germany.

Archives of Virology
|August 18, 2015
PubMed
Summary

A new genotyping method for herpes simplex virus type 1 (HSV-1) incorporates the US2 gene, improving classification. Genotype A of the US2 gene is more prevalent in women with herpes labialis.

More Related Videos

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins
08:26

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Published on: August 31, 2017

14.3K
Plaquing of Herpes Simplex Viruses
04:41

Plaquing of Herpes Simplex Viruses

Published on: November 5, 2021

7.3K

Related Experiment Videos

Last Updated: Apr 5, 2026

Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining
13:22

Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining

Published on: January 23, 2014

18.8K
Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins
08:26

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Published on: August 31, 2017

14.3K
Plaquing of Herpes Simplex Viruses
04:41

Plaquing of Herpes Simplex Viruses

Published on: November 5, 2021

7.3K

Area of Science:

  • Virology
  • Molecular Biology
  • Genetics

Background:

  • Herpes simplex virus type 1 (HSV-1) has three known genotypes (A, B, C) based on US4 and US7 gene polymorphisms.
  • Current genotyping methods for HSV-1 may not fully capture viral diversity.

Purpose of the Study:

  • To propose and validate an expanded genotyping method for HSV-1 that includes polymorphisms in the US2 gene.
  • To analyze the distribution of new HSV-1 genotypes in clinical isolates and assess their correlation with existing genotypes and clinical presentation.

Main Methods:

  • A refined genotyping method was developed incorporating US2 gene polymorphisms.
  • The method was validated using 423 clinical isolates from patients with various HSV-1 diseases.
  • Statistical analyses were performed to correlate US2 genotypes with US4/US7 genotypes and clinical data.

Main Results:

  • The proportions of US2 genotypes in the clinical isolates were determined: Genotype A (46.6%), Genotype B (23.2%), and Genotype C (30.2%).
  • A significant correlation was observed between US2 Genotype A and the previously defined US4/US7 genotypes.
  • Genotype A of US2 was found significantly more frequently in women than in men diagnosed with herpes labialis.

Conclusions:

  • The inclusion of US2 gene polymorphisms refines HSV-1 genotyping, offering a more comprehensive classification.
  • The US2 genotyping method demonstrates a strong correlation with established HSV-1 genotypes.
  • US2 Genotype A may be associated with a higher susceptibility or different disease manifestation in women with herpes labialis.