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High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging
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A cost-effective fluorescence mini-microscope for biomedical applications.

Yu Shrike Zhang1, João Ribas, Akhtar Nadhman

  • 1Biomaterials Innovation Research Center, Division of Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA. alik@rics.bwh.harvard.edu.

Lab on a Chip
|August 19, 2015
PubMed
Summary
This summary is machine-generated.

We developed a low-cost, miniature fluorescence microscope using readily available parts. This versatile device enables real-time monitoring of cell viability and microenvironment properties for various biomedical applications.

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Area of Science:

  • Biomedical Engineering
  • Microscopy
  • Cell Biology

Background:

  • Conventional microscopy can be expensive and cumbersome for long-term, in situ analysis.
  • There is a need for accessible, versatile imaging tools in cell biology and tissue engineering.

Purpose of the Study:

  • To design and fabricate a low-cost miniature fluorescence microscope.
  • To demonstrate its utility in assessing biochemical and biophysical parameters in biological samples.

Main Methods:

  • Constructed a miniature microscope using off-the-shelf components and a webcam.
  • Integrated fluorescence capability for biochemical and biophysical measurements.
  • Tested adjustable magnification (8-60×), resolution (<2 μm), and working distance (4.5 mm).

Main Results:

  • Successfully detected cell/tissue viability and oxygen levels in microfabricated tissues.
  • Monitored cell migration and analyzed beating in microfluidic bioreactors in real time.
  • Demonstrated adjustable magnification, high resolution, and long working distance.

Conclusions:

  • The developed mini-microscope is a cost-effective, modular tool for biomedical imaging.
  • It offers potential to replace conventional microscopy for long-term, in situ, and large-scale analyses.
  • Envisioned applications span cell biology, tissue engineering, biosensing, and organs-on-chips.