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Updated: Apr 5, 2026

Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice
Published on: January 6, 2018
Where is the common ground between bone marrow mesenchymal stem/stromal cells from different donors and species?
Elena Jones1, Richard Schäfer2
1Leeds Institute of Rheumatic and Musculoskeletal Medicine, Leeds University, Room 5.24 Clinical Sciences Building, St James's University Hospital, Leeds, LS9 7TF, UK. msjej@leeds.ac.uk.
Abstract:
Mesenchymal stem/stromal cells (MSCs) feature promising potential for cellular therapies, yet significant progress in development of MSC therapeutics and assays is hampered because of remarkable MSC heterogeneity in vivo and in vitro. This heterogeneity poses challenges for standardization of MSC characterization and potency assays as well as for MSC study comparability and manufacturing. This review discusses promising marker combinations for prospective MSC subpopulation enrichment and expansion, and reflects MSC phenotype changes due to environment and age. In order to address animal modelling in MSC biology, comparison of mouse and human MSC markers highlights current common ground of MSCs between species.
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