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High-Resolution Mass Spectrometry (HRMS)

High-Resolution Mass Spectrometry (HRMS)

The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...

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Related Experiment Video

Updated: Apr 5, 2026

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

Published on: April 17, 2018

Histamine quantification in human plasma using high resolution accurate mass LC-MS technology.

Mathieu Laurichesse¹, Thomas Gicquel², Caroline Moreau³

¹CHU Rennes, Laboratoire d'Immunologie, Thérapie Cellulaire et Hématopoïèse, F-35033 Rennes, France; CHU Rennes, Laboratoire de Biochimie, F-35033 Rennes, France.

Clinical Biochemistry

|August 19, 2015

Summary

A new liquid chromatography-high resolution mass spectrometry (LCHRMS) method accurately quantifies histamine in human plasma. This sensitive LCHRMS technique offers a faster, safer alternative to traditional radioimmunoassay for clinical diagnostics.

Keywords:

Accurate mass Anaphylactic reaction Histamine LC–high resolution-MS

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Published on: January 12, 2024

Area of Science:

Analytical Chemistry
Biochemistry
Clinical Diagnostics

Background:

Histamine (HA) is a key mediator in anaphylactic reactions.
Existing methods for HA quantification in plasma have limitations.
Development of a novel LCHRMS method using Q Exactive™.

Purpose of the Study:

To develop and validate a sensitive LCHRMS method for histamine quantification in human plasma.
To establish an alternative to existing, less advantageous methods.
To assess the suitability of LCHRMS for routine clinical laboratory use.

Main Methods:

Protein precipitation of plasma samples with deuterated histamine-d4 as internal standard.
LC separation on a C18 Accucore column with a gradient mobile phase.
Analysis using full scan and targeted MS2 modes with specific ion transitions.

Main Results:

Successful separation and quantification of histamine within 10 minutes.
Method validation across a concentration range of 1-100 nM with <15% precision.
High correlation of LCHRMS results with radioimmunoassay (RIA) in patient samples.

Conclusions:

LCHRMS is a sensitive and reliable method for histamine quantification in human plasma.
The method is suitable for routine use in medical laboratories.
LCHRMS offers advantages over RIA, including speed and avoidance of radioactivity.