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A rapid procedure for the purification of IgA1 and IgA2 subclasses from normal human serum using protein G and
1Protein Chemistry Section, Canadian Red Cross Society, Ottawa, Ontario.
Immunology Letters
|October 1, 1989
Abstract:
Immunoglobulin A (IgA) from pooled normal human sera was purified using antibody and protein G affinity chromatography and gel filtration high-pressure liquid chromatography (HPLC). This high purity product was separated into IgA1 and IgA2 subclasses utilizing the agarose-bound lectin 'jacalin'. Evaluation of product homogeneity by immunological testing confirmed greater than 95% purity. The total IgA1 and IgA2 recovered from sera was approximately 26% of the initial antibody present.