Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Apr 5, 2026

Automated Sholl Analysis of Digitized Neuronal Morphology at Multiple Scales
11:41

Automated Sholl Analysis of Digitized Neuronal Morphology at Multiple Scales

Published on: November 14, 2010

34.5K

Assessing effects on dendritic arborization using novel Sholl analyses.

Kate M O'Neill1, Barbara F Akum2, Survandita T Dhawan2

  • 1Department of Cell Biology and Neuroscience, Rutgers University Piscataway, NJ, USA ; Graduate Program in Biomedical Engineering, Rutgers University Piscataway, NJ, USA.

Frontiers in Cellular Neuroscience
|August 19, 2015
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Uric Acid Released from Poly (Lactic-co-Glycolic Acid) Nanoparticles Mitigates Glutamate-Induced Excitotoxicity of Spinal Cord Neurons.

ASN neuro·2026
Same author

Advancing molecular and cellular neuroscience: Vision and priorities from the new editor-in-chief.

Molecular and cellular neurosciences·2026
Same author

Network-Level Characterization of Spontaneous Calcium Activity in an In-Vitro Alzheimer's Disease Model.

bioRxiv : the preprint server for biology·2026
Same author

Corrigendum to "Caffeine inhibits hypoxia-induced nuclear accumulation in HIF-1α and promotes neonatal neuronal survival" [Experimental Neurology, 317 (2019) 66-77].

Experimental neurology·2026
Same author

Differential <i>SP4</i> expression and HSP60 abundance in buccal swabs from patients with schizophrenia.

Science advances·2026
Same author

Early Operative Management of Ballistic Humeral Shaft Fractures Is Associated With Shorter and More Predictable Course of Healing.

Orthopedics·2026

Standard methods for analyzing dendritic arbors miss local changes. Our study reveals novel insights into dendritic morphology regulation by cytosolic PSD-95 interactor (cypin) using advanced Sholl analysis.

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Cell Biology

Background:

  • Dendritic arbor shape is crucial for neural circuitry and is tightly regulated during development.
  • Aberrant dendritic morphology is linked to neurological diseases and injuries.
  • Current methods like Sholl analysis and dendrite counting often fail to detect localized changes in dendritic structure.

Purpose of the Study:

  • To investigate previously unknown changes in dendritic arbors.
  • To assess the impact of overexpressing cytosolic PSD-95 interactor (cypin) on dendritic morphology at two developmental stages.
  • To evaluate the sufficiency of standard Sholl analysis and dendrite counting versus novel approaches.

Main Methods:

  • Development and application of the Bonfire software for neurite morphology digitization and Sholl analysis.
Keywords:
Sholldendriteimage analysismorphologyneurontracing

More Related Videos

Assessment of Hippocampal Dendritic Complexity in Aged Mice Using the Golgi-Cox Method
09:44

Assessment of Hippocampal Dendritic Complexity in Aged Mice Using the Golgi-Cox Method

Published on: June 22, 2017

15.8K
Quantitative Analysis of Neuronal Dendritic Arborization Complexity in Drosophila
07:13

Quantitative Analysis of Neuronal Dendritic Arborization Complexity in Drosophila

Published on: January 7, 2019

14.8K

Related Experiment Videos

Last Updated: Apr 5, 2026

Automated Sholl Analysis of Digitized Neuronal Morphology at Multiple Scales
11:41

Automated Sholl Analysis of Digitized Neuronal Morphology at Multiple Scales

Published on: November 14, 2010

34.5K
Assessment of Hippocampal Dendritic Complexity in Aged Mice Using the Golgi-Cox Method
09:44

Assessment of Hippocampal Dendritic Complexity in Aged Mice Using the Golgi-Cox Method

Published on: June 22, 2017

15.8K
Quantitative Analysis of Neuronal Dendritic Arborization Complexity in Drosophila
07:13

Quantitative Analysis of Neuronal Dendritic Arborization Complexity in Drosophila

Published on: January 7, 2019

14.8K
  • Utilizing standard Sholl analysis, dendrite counting, and a novel Sholl analysis method.
  • Overexpression of cytosolic PSD-95 interactor (cypin) in a developmental model.

    • Main Results:

    • Overexpression of cypin led to previously undetected local changes in dendritic arbors.
    • Standard Sholl analysis and simple dendrite counting were insufficient to reveal these localized alterations.
    • The novel Sholl analysis approach successfully identified specific changes in dendritic morphology.

    Conclusions:

    • Standard methods for analyzing dendritic morphology have limitations in detecting local changes.
    • Advanced Sholl analysis techniques are necessary for a comprehensive understanding of dendritic arbor regulation.
    • Cytosolic PSD-95 interactor (cypin) plays a significant role in regulating local dendritic structure during development.