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Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity
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Transcriptome-wide dynamics of RNA pseudouridylation

John Karijolich1, Chengqi Yi2, Yi-Tao Yu3

  • 1Department of Plant and Microbial Biology, University of California, 565 Li Ka Shing Center #3370, Berkeley, California 94720-337, USA.

Nature Reviews. Molecular Cell Biology
|August 20, 2015
PubMed
Summary

Pseudouridylation, a key RNA modification, is now mapped across the transcriptome, revealing its role in cellular stress and on messenger RNAs (mRNAs). This discovery expands our understanding of RNA pseudouridylation and its impact on protein complexity.

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