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Updated: Apr 5, 2026
Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma
Published on: March 13, 2013
Comparative gene-expression profiling of CD133(+) and CD133(-) D10 melanoma cells
Raheleh Roudi1,2, Marzieh Ebrahimi3, Mehrdad Nasrollahzadeh Sabet2
1Oncopathology Research Center, Iran University of Medical Sciences, Hemmat Street (Highway), Next to Milad Tower, Tehran 14496-14530, Iran.
Aims:
The present study aimed to compare the gene-expression profiling of CD133(+) and CD133(-) D10 cells.
Materials & Methods:
Cancer stem cell-like properties and gene-expression profiling of CD133(+) D10 cells versus CD133(-) cells were evaluated.
Results:
The CD133(+) D10 cells showed significantly higher clonogenic and spheroid forming potential, also higher expression of stemness genes NANOG and OCT4A compared with the CD133(-) cells. Gene-expression profiling of CD133(+) versus CD133(-) D10 cells revealed that 130 genes including ABC transporter superfamily (ABCC1, ABCG2 and ABCC6) were upregulated, while 61 genes including apoptosis modifying genes (CASP8 and TNFRSF4) were downregulated.
Conclusion:
We conclude that many genes involved in drug resistance and tumor aggressiveness are upregulated in CD133(+) D10 cells and targeting them might be an efficient strategy for treatment of melanoma.
