Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Methods of Classification and Identification

Methods of Classification and Identification

Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...

Methods to Assess Microbial Populations

Methods to Assess Microbial Populations

Assessing microbial populations is crucial for understanding microbial roles in health, ecology, and industry. Various complementary techniques—both culture-based and molecular—enable detailed analysis of microbial abundance, diversity, and function.Viable Plate CountThe viable plate count is a traditional culture-based method used to estimate the number of living microbes in a sample. After serial dilution, the sample is spread onto nutrient agar plates. Each viable cell forms a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

An electrochemical biosensor for the detection of microRNA-31 as a potential oral cancer biomarker.

Journal of biological engineering·2025

Same author

Perspectives on the Application of Biosensors for the Early Detection of Oral Cancer.

Sensors (Basel, Switzerland)·2025

Same author

A Cell-Based Electrochemical Biosensor for the Detection of Infectious Hepatitis A Virus.

Biosensors·2024

Same author

An Electrochemical Nucleic Acid Biosensor for Triple-Negative Breast Cancer Biomarker Detection.

Sensors (Basel, Switzerland)·2024

Same author

Current advances in Hepatitis C diagnostics.

Journal of biological engineering·2024

Same author

A Bacteriophage Protein-Based Impedimetric Electrochemical Biosensor for the Detection of <i>Campylobacter jejuni</i>.

Biosensors·2024

Search research articles

Related Experiment Video

Updated: Apr 5, 2026

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System

Published on: February 23, 2018

Current and Prospective Methods for Plant Disease Detection.

Yi Fang¹, Ramaraja P Ramasamy²

¹Nano Electrochemistry Laboratory, College of Engineering, University of Georgia, Athens, GA 30602, USA. fangyi@uga.edu.

|August 20, 2015

Summary

This summary is machine-generated.

Advanced crop disease detection is crucial for sustainable agriculture. This review covers direct and indirect methods, including biosensors, for early identification and prevention of crop infections.

Keywords:

DNA/RNA antibody bacteriophage enzyme food loss plant pathogen sensor volatile organic compounds

More Related Videos

A Rapid and Efficient Method for Assessing Pathogenicity of Ustilago maydis on Maize and Teosinte Lines

A Rapid and Efficient Method for Assessing Pathogenicity of Ustilago maydis on Maize and Teosinte Lines

Published on: January 3, 2014

Author Spotlight: A Streamlined Approach to Studying Cell Death Initiation in Hypersensitive Response

Author Spotlight: A Streamlined Approach to Studying Cell Death Initiation in Hypersensitive Response

Published on: November 10, 2023

Related Experiment Videos

Last Updated: Apr 5, 2026

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System

Published on: February 23, 2018

A Rapid and Efficient Method for Assessing Pathogenicity of Ustilago maydis on Maize and Teosinte Lines

A Rapid and Efficient Method for Assessing Pathogenicity of Ustilago maydis on Maize and Teosinte Lines

Published on: January 3, 2014

Author Spotlight: A Streamlined Approach to Studying Cell Death Initiation in Hypersensitive Response

Author Spotlight: A Streamlined Approach to Studying Cell Death Initiation in Hypersensitive Response

Published on: November 10, 2023

Area of Science:

Agricultural Science
Plant Pathology
Biotechnology

Background:

Crop infections by bacteria, viruses, and fungi cause significant global food losses.
Minimizing disease-induced damage is essential for agricultural productivity and sustainability.
Advanced disease detection and prevention are imperative throughout the crop lifecycle.

Purpose of the Study:

To review current direct and indirect methods for crop disease identification.
To provide an overview of emerging biosensor technologies for early disease detection.
To highlight the importance of advanced diagnostics for agricultural sustainability.

Main Methods:

Review of laboratory-based direct detection techniques: Polymerase Chain Reaction (PCR), Immunofluorescence (IF), Fluorescence In-Situ Hybridization (FISH), Enzyme-Linked Immunosorbent Assay (ELISA), Flow Cytometry (FCM), Gas Chromatography-Mass Spectrometry (GC-MS).
Review of indirect detection methods: Thermography, Fluorescence Imaging, Hyperspectral Imaging.
Overview of biosensors utilizing bio-recognition elements (enzymes, antibodies, DNA/RNA, bacteriophages).

Main Results:

Direct methods offer precise pathogen identification but often require laboratory settings.
Indirect methods provide non-invasive, large-scale monitoring capabilities.
Biosensors represent a promising tool for rapid, early, and on-site crop disease diagnosis.

Conclusions:

A combination of direct, indirect, and biosensor-based methods is needed for comprehensive crop disease management.
Early detection through advanced techniques is key to reducing food losses and enhancing agricultural sustainability.
Biosensors offer a novel approach for timely intervention against crop pathogens.