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Updated: Apr 5, 2026

Mimicking and Measuring Occlusal Erosive Tooth Wear with the "Rub&Roll" and Non-contact Profilometry
Published on: February 2, 2018
Effect of Model Variables on in vitro Erosion
Miten Mistry1, Shanshan Zhu, Rebecca Moazzez
1King's College London, London, UK.
Tooth type, surface, and smear layer significantly impact enamel erosion and microhardness. Agitation speed and mode also affect step height loss, emphasizing the need for detailed methods in dental research.
Area of Science:
- Dental materials science
- Biomaterials engineering
- Surface analysis
Background:
- Dental erosion is a complex process influenced by various factors.
- Understanding these factors is crucial for developing effective preventive and restorative strategies.
- Standardized methodologies are essential for reproducible research in dental erosion.
Purpose of the Study:
- To investigate the influence of tooth type, surface, smear layer presence, storage, agitation, and rinsing on enamel erosion.
- To quantify changes in mean step height loss and Knoop microhardness (KHN) under different experimental conditions.
- To highlight the importance of precise methodological details in dental erosion studies.
Main Methods:
- Human molar and premolar enamel specimens were prepared and subjected to citric acid erosion in a cycling procedure.
- Specimens were analyzed for mean step height loss using white light profilometry and Knoop microhardness (KHN).
- Variables included tooth type, surface, smear layer, storage, agitation (mode and speed), and rinsing methods.
Main Results:
- Molar teeth and buccal surfaces showed significantly less microhardness change compared to premolars and lingual surfaces.
- Absence of a smear layer resulted in significantly lower step height loss and microhardness change.
- Orbital agitation at 30 rpm yielded the lowest step height loss, while See-Saw agitation at 70 rpm yielded the highest.
Conclusions:
- Tooth type, surface characteristics, and the presence or absence of a smear layer significantly influence enamel erosion and microhardness.
- Agitation mode and speed are critical variables affecting the measurable outcomes of dental erosion.
- Accurate and detailed reporting of experimental methods is paramount for the reliability and reproducibility of dental erosion research.
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