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Published on: April 25, 2025
Antimicrobial activities of Saudi honey against Pseudomonas aeruginosa
Alaa A M Al-Nahari1, Saad B Almasaudi1, El Sayed M Abd El-Ghany2
1Biology Department, Faculty of Science, King Abdulaziz University (KAU), Saudi Arabia.
Unlabelled:
Five types of imported and local honey were screened for both their bacteriocidal/bacteriostatic activities against both Imipenem resistant and sensitive Pseudomonas aeruginosa in both Brain Heart infusion broth and Mueller-Hinton agar. The results indicated that the effect was concentration and type of honey dependant. All types of honey tested exerted a full inhibition of bacterial growth at the highest concentration tested of 50% at 24 h of contact. The inhibitory effect of honey on bacterial growth was clear with concentrations of 20% and 10% and this effect was most evident in the case of Manuka honey as compared to Nigella sativa honey and Seder honey. Manuka honey UMF +20 showed a bacteriocidal activity on both Imipenem resistant and sensitive P. aeruginosa, while Seder honey and N. sativa honey exerted only a bacteriostatic effect. Manuka honey UMF +10 showed most effect on antimicrobial resistance. Manuka honey UMF +10 had an effect on modulation of Imipenem resistant P. aeruginosa.
Conclusion:
The results indicated that various types of honey affected the test organisms differently. Modulation of antimicrobial resistance was seen in the case Manuka honey UMF +10.
Insights
Manuka honey demonstrates significant antibacterial activity against Pseudomonas aeruginosa, including resistant strains. Certain honey types, like Manuka honey UMF +10, show potential in modulating antimicrobial resistance.
Area of Science:
- Microbiology
- Natural Products Chemistry
- Pharmacology
Background:
- Pseudomonas aeruginosa is a significant opportunistic pathogen, often exhibiting multidrug resistance.
- Honey possesses known antimicrobial properties, but its efficacy against resistant bacterial strains requires further investigation.
Purpose of the Study:
- To evaluate the in vitro antibacterial activity of five honey types against imipenem-resistant and sensitive Pseudomonas aeruginosa.
- To determine the concentration-dependent effects and specific activities (bactericidal vs. bacteriostatic) of different honeys.
Main Methods:
- Screening of five honey types for antibacterial activity using Brain Heart Infusion broth and Mueller-Hinton agar.
- Testing against both imipenem-resistant and sensitive strains of Pseudomonas aeruginosa.
- Assessing dose-dependent effects and contact time (24 hours).
Main Results:
- All tested honeys inhibited bacterial growth at 50% concentration.
- Manuka honey (UMF +20) exhibited bactericidal activity against both resistant and sensitive P. aeruginosa.
- Manuka honey (UMF +10) demonstrated the most significant impact on antimicrobial resistance and modulated imipenem resistance.
Conclusions:
- Honey exhibits variable antibacterial effects against Pseudomonas aeruginosa, dependent on honey type and concentration.
- Manuka honey, particularly UMF +10, shows promise in combating antimicrobial resistance and modulating bacterial response to antibiotics.
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