Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A smarter dermal filler: Antimicrobial protection and collagen stimulation with niacinamide.

European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V·2026
Same author

Protein-based nanocarrier delivering kartogenin derivative to cartilage matrix for intra-articular treatment of osteoarthritis.

Journal of drug delivery science and technology·2026
Same author

Stepwise effects of formulation additives and process parameters on ethanol injection-prepared nanoliposomes for cancer delivery: a preformulation study.

Scientific reports·2026
Same author

Lipidomic profiling of extracellular vesicles from breast and metastatic triple-negative breast cancer cell lines for identification of potential biomarkers.

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences·2026
Same author

Fully synthetic, nature-inspired exosome-mimetics for melanoma therapy.

International journal of pharmaceutics·2026
Same author

Enzymatically-responsive hyaluronan-glucose hydrogel supports MSC survival and preserves paracrine function under glucose deprivation.

International journal of pharmaceutics·2026

Related Experiment Video

Updated: Apr 5, 2026

Microwave-assisted Functionalization of Polyethylene glycol and On-resin Peptides for Use in Chain Polymerizations and Hydrogel Formation
15:33

Microwave-assisted Functionalization of Polyethylene glycol and On-resin Peptides for Use in Chain Polymerizations and Hydrogel Formation

Published on: October 29, 2013

29.9K

Sustained protein release from hydrogel microparticles using layer-by-layer (LbL) technology.

Omar S Sakr1, Olivier Jordan1, Gerrit Borchard1

  • 1a School of Pharmaceutical Sciences Geneva-Lausanne, University of Geneva, University of Lausanne , Geneva , Switzerland.

Drug Delivery
|August 21, 2015
PubMed
Summary

This study developed a hydrogel microparticle formulation using layer-by-layer coating to achieve sustained release of lysozyme (Lys). The formulation successfully maintained protein activity for a month, showing potential for therapeutic protein delivery.

Keywords:
Layer-by-layerlysozymeprotein activityprotein deliverysustained delivery

More Related Videos

Fabricating Degradable Thermoresponsive Hydrogels on Multiple Length Scales via Reactive Extrusion, Microfluidics, Self-assembly, and Electrospinning
12:07

Fabricating Degradable Thermoresponsive Hydrogels on Multiple Length Scales via Reactive Extrusion, Microfluidics, Self-assembly, and Electrospinning

Published on: April 16, 2018

14.2K
Alternating Magnetic Field-Responsive Hybrid Gelatin Microgels for Controlled Drug Release
09:11

Alternating Magnetic Field-Responsive Hybrid Gelatin Microgels for Controlled Drug Release

Published on: February 13, 2016

10.5K

Related Experiment Videos

Last Updated: Apr 5, 2026

Microwave-assisted Functionalization of Polyethylene glycol and On-resin Peptides for Use in Chain Polymerizations and Hydrogel Formation
15:33

Microwave-assisted Functionalization of Polyethylene glycol and On-resin Peptides for Use in Chain Polymerizations and Hydrogel Formation

Published on: October 29, 2013

29.9K
Fabricating Degradable Thermoresponsive Hydrogels on Multiple Length Scales via Reactive Extrusion, Microfluidics, Self-assembly, and Electrospinning
12:07

Fabricating Degradable Thermoresponsive Hydrogels on Multiple Length Scales via Reactive Extrusion, Microfluidics, Self-assembly, and Electrospinning

Published on: April 16, 2018

14.2K
Alternating Magnetic Field-Responsive Hybrid Gelatin Microgels for Controlled Drug Release
09:11

Alternating Magnetic Field-Responsive Hybrid Gelatin Microgels for Controlled Drug Release

Published on: February 13, 2016

10.5K

Area of Science:

  • Biomaterials Science
  • Drug Delivery
  • Protein Engineering

Background:

  • Therapeutic proteins often require long-term administration for chronic diseases, necessitating sustained-release formulations.
  • Current challenges in protein delivery include maintaining therapeutic efficacy and controlling release kinetics.
  • Hydrogel microparticles offer a promising platform for encapsulating and delivering protein therapeutics.

Purpose of the Study:

  • To investigate the efficacy of layer-by-layer (LbL) coating on hydrogel microparticles for controlled release of lysozyme (Lys).
  • To evaluate the ability of LbL-coated hydrogel beads to maintain the biological activity of loaded lysozyme over an extended period.
  • To explore the potential of this formulation for sustained delivery of therapeutic proteins.

Main Methods:

  • Lysozyme (Lys) was loaded onto hydrogel microparticles.
  • Layer-by-layer (LbL) coating using chondroitin sulfate and a poly β-aminoester was applied to the microparticles.
  • Coating thickness was varied, and loading distribution was confirmed using fluorescence imaging.
  • In vitro release studies and biological activity assays were performed on the released Lys.

Main Results:

  • Effective loading of Lys onto hydrogel beads was achieved (9 mg protein/100 mg wet spheres).
  • LbL coating was confirmed by zeta potential measurements, showing successful layer deposition.
  • Sustained release profiles were observed, with release duration dependent on coating thickness (t50 extended from 4.9 to 143.9 h).
  • Released Lys retained significant biological activity, with at least 72% of initial activity maintained.

Conclusions:

  • The developed LbL-coated hydrogel microparticle formulation enables effective loading and sustained release of lysozyme.
  • The formulation successfully maintains the biological activity of the therapeutic protein over time.
  • This approach shows promise for the development of advanced drug delivery systems for various therapeutic proteins.