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Related Experiment Video

Updated: Apr 5, 2026

Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors

Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors

Published on: August 7, 2021

A high-throughput acoustic cell sorter.

Liqiang Ren¹, Yuchao Chen¹, Peng Li¹

¹Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802, USA.

|August 21, 2015

Summary

This summary is machine-generated.

This study introduces a high-throughput cell sorting method using standing surface acoustic waves (SSAWs), significantly improving sorting rates for acoustic cell sorters. The new method achieves over 13,800 events per second, advancing acoustic-based fluorescence activated cell sorting (FACS) applications.

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Last Updated: Apr 5, 2026

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Area of Science:

Biotechnology
Acoustic physics
Cell sorting technology

Background:

Acoustic-based fluorescence activated cell sorters (FACS) offer advantages like biocompatibility and controllability.
Current acoustic cell sorters face limitations in sorting throughput, hindering practical applications.

Purpose of the Study:

To develop a high-throughput cell sorting method using standing surface acoustic waves (SSAWs).
To enhance the sorting efficiency and practical applicability of acoustic-based cell sorting.

Main Methods:

Generation of SSAWs using a pair of focused interdigital transducers (FIDTs) for high resolution and energy efficiency.
Demonstration of particle sorting with precise actuation time control.

Main Results:

Achieved a potential sorting rate exceeding 13,800 events per second for 10 μm polystyrene particles.
Demonstrated an actual sorting throughput of 3300 events per second without a cell-detection unit.
Significant improvement in sorting throughput compared to conventional acoustic methods.

Conclusions:

The SSAW-based method offers a substantial advancement in acoustic cell sorting throughput.
This technique is compatible with upstream detection units, paving the way for functional acoustic-based FACS systems.