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Identification of recombinant human EPO variants in greyhound plasma and urine by ELISA, LC-MS/MS and western
Mark Timms1, Rohan Steel1, John Vine1
1Biological Research Unit, Racing Analytical Services Ltd, 400 Epsom Road, Flemington, Victoria, 3031, Australia.
Abstract:
The recombinant human erythropoietins epoetin alfa (Eprex®), darbepoetin (Aranesp®) and methoxy polyethylene glycol-epoetin beta (Mircera®) were administered to greyhounds for 7, 10 and 14 days respectively. Blood and urine samples were collected and analysed for erythropoietin by ELISA, LC-MS/MS and western blotting. Limits of confirmation in plasma for western blotting and LC-MS/MS methods ranged from a low of 2.5mIU/mL, and closely matched the sensitivity of ELISA screening.
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