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Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
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Boosting Dissolution Dynamic Nuclear Polarization by Cross Polarization.

Aurélien Bornet1, Roberto Melzi2, Angel J Perez Linde1

  • 1†Institut des Sciences et Ingénierie Chimiques, Ecole Polytechnique Fédérale de Lausanne (EPFL), Batochime, CH-1015 Lausanne, Switzerland.

The Journal of Physical Chemistry Letters
|August 21, 2015
PubMed
Summary
This summary is machine-generated.

Hartmann-Hahn cross polarization near 1.2 K boosts dissolution dynamic nuclear polarization efficiency. This method enhances hyperpolarized solution throughput, significantly reducing buildup times and improving polarization levels.

Keywords:
TEMPOcross polarizationdissolution DNPhyperpolarizationradicals

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Area of Science:

  • Nuclear Magnetic Resonance (NMR) spectroscopy
  • Quantum sensing
  • Biophysics

Background:

  • Dynamic nuclear polarization (DNP) is crucial for enhancing NMR signal sensitivity.
  • Dissolution DNP (dDNP) allows for rapid transfer of hyperpolarized agents for in vivo imaging.
  • Maintaining high polarization levels during dissolution and transport is a key challenge.

Purpose of the Study:

  • To investigate the efficiency of Hartmann-Hahn cross polarization for boosting dDNP.
  • To assess the impact of low temperatures (near 1.2 K) on polarization levels.
  • To evaluate the feasibility of high-throughput production of hyperpolarized solutions.

Main Methods:

  • Utilized Hartmann-Hahn cross polarization technique.
  • Operated at cryogenic temperatures around 1.2 K.
  • Measured (13)C nuclear spin polarization (P((13)C)) before and after dissolution/transport.

Main Results:

  • Achieved boosted efficiency in dissolution DNP.
  • Demonstrated substantial gains in buildup times and polarization levels.
  • Observed minimal decrease in (13)C polarization (from 45% to 40%) during dissolution and transport.

Conclusions:

  • Hartmann-Hahn cross polarization is an effective method to enhance dDNP efficiency at low temperatures.
  • The technique enables high-throughput production of hyperpolarized solutions with preserved polarization.
  • This advancement has significant implications for accelerating NMR-based research and diagnostics.