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Related Experiment Video

Updated: Apr 5, 2026

A General Method for Evaluating Deep Brain Stimulation Effects on Intravenous Methamphetamine Self-Administration

A General Method for Evaluating Deep Brain Stimulation Effects on Intravenous Methamphetamine Self-Administration

Published on: January 22, 2016

Role for Rab10 in Methamphetamine-Induced Behavior.

Scott M Vanderwerf¹, David C Buck², Phillip A Wilmarth³

¹Department of Behavioral Neuroscience, Oregon Health & Science University, Portland, Oregon, United States of America; Department of Integrative Biosciences, Oregon Health & Science University, Portland, Oregon, United States of America.

|August 21, 2015

Summary

This summary is machine-generated.

Methamphetamine alters proteins within specialized cell membrane compartments called lipid rafts. This study identifies Rab10 protein redistribution as critical for psychostimulant-induced behaviors and mortality.

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Area of Science:

Neuroscience
Cell Biology
Pharmacology

Background:

Lipid rafts are crucial for organizing cell signaling pathways.
Understanding how abused psychostimulants like methamphetamine affect these rafts is key to deciphering their acute and chronic effects.

Purpose of the Study:

To identify proteins in lipid rafts whose abundance changes following methamphetamine treatment.
To investigate the role of altered raft proteins in psychostimulant-induced behaviors.

Main Methods:

Detergent-free preparation of rat brain striatal membrane rafts.
Liquid chromatography-mass spectrometry (LC-MS) for protein identification.
Functional studies using a dominant-negative Rab10 mutant in Drosophila melanogaster.

Main Results:

Methamphetamine treatment altered the relative abundance of 23 raft proteins.
Rab10 protein abundance in rafts decreased significantly after acute methamphetamine exposure.
Inhibition of Rab10 activity in Drosophila reduced methamphetamine-induced activity and mortality.

Conclusions:

Rab10 redistribution within lipid rafts is implicated in psychostimulant effects.
Rab10 plays a critical role in mediating certain behavioral responses to methamphetamine.