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Related Experiment Video

Updated: Apr 5, 2026

Intramyocardial Cell Delivery: Observations in Murine Hearts

Intramyocardial Cell Delivery: Observations in Murine Hearts

Published on: January 24, 2014

Human Engineered Heart Muscles Engraft and Survive Long Term in a Rodent Myocardial Infarction Model.

Johannes Riegler¹, Malte Tiburcy¹, Antje Ebert¹

¹From the Division of Cardiology, Department of Medicine, Stanford Cardiovascular Institute (J.R., A.E., E.T., U.R., O.J.A., O.S., N.G.K., E.N., M.W., P.S.T., J.D.G., J.C.W.) and Department of Pathology (A.J.C.), Stanford University School of Medicine, CA; Department for Research and Development, Veterans Administration Palo Alto Health Care System, CA (P.S.T.); Institute of Pharmacology, Heart Research Center, University Medical Center, Georg-August-University and German Center for Cardiovascular Research, Göttingen, Germany (M.T., T.M., W.H.Z.); and Center for Biomedicine and Genetics (V.C.C., L.A.C.) and Center for Applied Technology Development, Beckman Research Institute (A.J.C.), City of Hope, Duarte, CA.

Circulation Research

|August 21, 2015

Summary

This summary is machine-generated.

Engineered heart muscle (EHM) transplantation shows promising long-term survival and maturation of human cardiomyocytes after myocardial infarction. However, engraftment did not improve function, though the approach demonstrated safety with no teratoma formation.

Keywords:

cardiac MRI cardiac function tests cell survival myocardial infarction myocardial ischemia tissue engineering transplantation

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Last Updated: Apr 5, 2026

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Cell-based Therapy for Heart Failure in Rat: Double Thoracotomy for Myocardial Infarction and Epicardial Implantation of Cells and Biomatrix

Published on: September 22, 2014

Area of Science:

Cardiovascular Research
Regenerative Medicine
Stem Cell Therapy

Background:

Tissue engineering offers potential for human embryonic stem cell-derived cardiomyocyte transplantation to improve cardiac function and prevent heart failure.
Engineered heart muscle (EHM) presents a promising approach for cell-based cardiac repair.

Purpose of the Study:

To assess the transport stability, survival, structural organization, functional benefits, and teratoma risk of EHM in a chronic myocardial infarction model.
To evaluate the efficacy of EHM transplantation in a preclinical setting.

Main Methods:

Human embryonic stem cell-derived cardiomyocytes were used to construct EHMs, which underwent transatlantic shipping.
EHMs were implanted onto immunocompromised rat hearts following induced ischemia/reperfusion injury.
Bioluminescence imaging and cardiac function assessments were performed to monitor engraftment and therapeutic effects.

Main Results:

Transatlantic shipping did not adversely affect EHM viability or function.
Stable engraftment of EHMs was observed for up to 12 weeks post-transplantation, with preserved cell viability.
Despite attenuated disease progression, EHM engraftment did not correlate with functional improvements; however, grafted cells showed structural maturation and no teratoma formation.

Conclusions:

EHM transplantation facilitates high engraftment rates, long-term survival, and maturation of human cardiomyocytes.
Functional benefits were not observed in this chronic myocardial infarction model, despite successful engraftment.
The safety of EHM transplantation was confirmed by the absence of tumor or teratoma formation.