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Multidrug-Resistance Related Long Non-Coding RNA Expression Profile Analysis of Gastric Cancer
Ying Wang1, Kaichun Wu2, Zhiping Yang2
1State Key Laboratory of Cancer Biology and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, 15 Changlexi Road, Xi'an, Shaanxi, China; Department of Oncology, First Affiliated Hospital of Henan University of Science and Technology, 24 Jinghua Road, Luoyang, Henan, China.
Abstract:
The effect of chemotherapy of gastric cancer (GC) remains very poor because of multidrug resistance (MDR). However, the mechanisms underlying MDR of GC remains far from fully understood. The aim of this study is to illustrate the potential mechanisms of the MDR of GC at mainly the long non-coding RNA (lncRNA) level. In this study, GC cell line, SGC7901, and two MDR sublines, SGC7901/VCR and SGC7901/ADR were subjected to an lncRNA microarray analysis. Bioinformatics and verification experiments were performed to investigate the potential lncRNAs involved in the development of MDR. Pathway analysis indicated that 15 pathways corresponded to down-regulated transcripts and that 20 pathways corresponded to up-regulated transcripts (p-value cut-off is 0.05). GO analysis showed that the highest enriched GOs targeted by up-regulated transcripts were "system development" and the highest esenriched GOs targeted by the down-regulated transcripts were "sterol biosynthetic process". Our study is the first to interrogate differentially expressed lncRNAs in human GC cell line and MDR sublines and indicates that lncRNAs are worthwhile for further study to be the novel candidate biomarkers for the clinical diagnosis of MDR and potential targets for further therapy.
Insights
This study explores long non-coding RNAs (lncRNAs) in gastric cancer (GC) multidrug resistance (MDR). Findings suggest lncRNAs play a role in GC MDR, offering potential new biomarkers and therapeutic targets.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Gastric cancer (GC) chemotherapy efficacy is limited by multidrug resistance (MDR).
- Mechanisms of GC MDR are not fully understood.
- Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in cancer progression and drug resistance.
Purpose of the Study:
- To investigate the potential mechanisms of GC MDR at the lncRNA level.
- To identify differentially expressed lncRNAs in GC MDR cell lines.
- To explore lncRNAs as potential biomarkers and therapeutic targets for GC MDR.
Main Methods:
- lncRNA microarray analysis of GC cell line SGC7901 and its MDR sublines (SGC7901/VCR, SGC7901/ADR).
- Bioinformatics analysis to identify differentially expressed lncRNAs.
- Verification experiments and pathway/Gene Ontology (GO) analysis.
Main Results:
- Identified differentially expressed lncRNAs between GC and MDR sublines.
- Pathway analysis revealed significant alterations in 15 down-regulated and 20 up-regulated pathways.
- GO analysis highlighted "system development" and "sterol biosynthetic process" as key targeted processes.
Conclusions:
- This study is the first to interrogate lncRNAs in human GC MDR cell lines.
- lncRNAs are implicated in the development of GC MDR.
- lncRNAs represent promising novel biomarkers for clinical diagnosis and potential therapeutic targets for GC MDR.
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