Related Experiment Video
Updated: Apr 5, 2026

Label-free in situ Imaging of Lignification in Plant Cell Walls
Published on: November 1, 2010
Vibrational fingerprint mapping reveals spatial distribution of functional groups of lignin in plant cell wall
Bin Liu1,2, Ping Wang2, Jeong Im Kim3
1National Key Laboratory of Science and Technology on Tunable Laser, Harbin Institute of Technology , Harbin 150080, China.
Abstract:
Highly lignified vascular plant cell walls represent the majority of cellulosic biomass. Complete release of the biomass to deliver renewable energy by physical, chemical, and biological pretreatments is challenging due to the "protection" provided by polymerized lignin, and as such, additional tools to monitor lignin deposition and removal during plant growth and biomass deconstruction would be of great value. We developed a hyperspectral stimulated Raman scattering microscope with 9 cm(-1) spectral resolution and submicrometer spatial resolution. Using this platform, we mapped the aromatic ring of lignin, aldehyde, and alcohol groups in lignified plant cell walls. By multivariate curve resolution of the hyperspectral images, we uncovered a spatially distinct distribution of aldehyde and alcohol groups in the thickened secondary cell wall. These results collectively contribute to a deeper understanding of lignin chemical composition in the plant cell wall.
More Related Videos
Related Concept Videos
IR Frequency Region: Fingerprint Region
IR Spectroscopy: Molecular Vibration Overview
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...
Plant Cell Wall
Plant Cell Wall
Cellulose and Pectic Polysaccharides
As a cell matures, its cell wall specializes according to its type. For example, the...
NMR Spectroscopy of Aromatic Compounds

