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Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle
Published on: July 11, 2015
Species preference of bovine thymocytes and fibroblasts for bovine interleukin 1
1Department of Pathobiological Sciences, University of Wisconsin, Madison 53706.
The purpose of this study was to determine whether bovine thymocytes and fibroblasts preferentially respond to bovine IL-1 as compared with human and murine interleukin 1 (IL-1). We compared equivalents units of recombinant murine and human IL-1 alpha, and purified monocyte-derived bovine IL-1, as measured in the murine thymocyte IL-1 assay, for their ability to stimulate the proliferation of bovine thymocytes and fibroblasts. Fetal and adult bovine thymocytes responded to bovine IL-1, but in contrast, did not respond to recombinant human or murine IL-1 alpha at the concentrations tested. Both adult and neonatal murine thymocytes responded similarly to the three IL-1 preparations. Thus, we concluded that the preferential response of bovine thymocytes for bovine IL-1 was not dependent upon the age of the thymus donor. Bovine fibroblasts also preferentially responded to bovine IL-1; fibroblast proliferation was stimulated by bovine but not human or murine IL-1. Our findings, therefore, imply that although IL-1 is highly conserved among mammalian species, its ability to stimulate the proliferation of thymocytes and fibroblasts may vary from species to species.
The purpose of this study was to determine whether bovine thymocytes and fibroblasts preferentially respond to bovine IL-1 as compared with human and murine interleukin 1 (IL-1). We compared equivalents units of recombinant murine and human IL-1 alpha, and purified monocyte-derived bovine IL-1, as measured in the murine thymocyte IL-1 assay, for their ability to stimulate the proliferation of bovine thymocytes and fibroblasts. Fetal and adult bovine thymocytes responded to bovine IL-1, but in contrast, did not respond to recombinant human or murine IL-1 alpha at the concentrations tested. Both adult and neonatal murine thymocytes responded similarly to the three IL-1 preparations. Thus, we concluded that the preferential response of bovine thymocytes for bovine IL-1 was not dependent upon the age of the thymus donor. Bovine fibroblasts also preferentially responded to bovine IL-1; fibroblast proliferation was stimulated by bovine but not human or murine IL-1. Our findings, therefore, imply that although IL-1 is highly conserved among mammalian species, its ability to stimulate the proliferation of thymocytes and fibroblasts may vary from species to species.
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