Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quantitative trait loci mapping provides insights into the genetic regulation of dendritic cell numbers in mouse tissues.

Cell reports·2024
Same author

State of the art and upcoming trends in HER2-directed therapies in gastrointestinal malignancies.

Current opinion in oncology·2024
Same author

Advances in Human Epidermal Growth Factor Receptor 2-Targeted Therapy in Upper Gastrointestinal Cancers.

Hematology/oncology clinics of North America·2024
Same author

Clonal Analysis of Human Dendritic Cell Progenitors Using a Stromal Cell Culture.

Methods in molecular biology (Clifton, N.J.)·2023
Same author

Distinct gene expression by expanded clones of quiescent memory CD4<sup>+</sup> T cells harboring intact latent HIV-1 proviruses.

Cell reports·2022
Same author

Reduced calcium levels and accumulation of abnormal insulin granules in stem cell models of HNF1A deficiency.

Communications biology·2022

Related Experiment Video

Updated: Apr 5, 2026

Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow
07:21

Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow

Published on: July 29, 2018

9.6K

Defining human dendritic cell progenitors by multiparametric flow cytometry.

Gaëlle Breton1, Jaeyop Lee2, Kang Liu2

  • 1Laboratory of Molecular Immunology, The Rockefeller University, New York, New York, USA.

Nature Protocols
|August 21, 2015
PubMed
Summary

This study details a rapid flow cytometry method to identify and isolate human dendritic cell (DC) progenitors from bone marrow and cord blood. These techniques aid in understanding DC development and function in various health and disease contexts.

More Related Videos

Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols
08:31

Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols

Published on: October 18, 2016

14.5K
Simultaneous Flow Cytometric Characterization of Multiple Cell Types Retrieved from Mouse Brain/Spinal Cord Through Different Homogenization Methods
10:24

Simultaneous Flow Cytometric Characterization of Multiple Cell Types Retrieved from Mouse Brain/Spinal Cord Through Different Homogenization Methods

Published on: November 19, 2019

14.5K

Related Experiment Videos

Last Updated: Apr 5, 2026

Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow
07:21

Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow

Published on: July 29, 2018

9.6K
Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols
08:31

Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols

Published on: October 18, 2016

14.5K
Simultaneous Flow Cytometric Characterization of Multiple Cell Types Retrieved from Mouse Brain/Spinal Cord Through Different Homogenization Methods
10:24

Simultaneous Flow Cytometric Characterization of Multiple Cell Types Retrieved from Mouse Brain/Spinal Cord Through Different Homogenization Methods

Published on: November 19, 2019

14.5K

Area of Science:

  • Immunology
  • Cell Biology
  • Hematopoiesis

Background:

  • Human dendritic cells (DCs) originate from restricted bone marrow (BM) progenitors.
  • Key progenitor populations include granulocyte, monocyte and DC progenitor (GMDP), monocyte and DC progenitor (MDP), common DC progenitor (CDP), and DC precursor (pre-DC) cells.
  • These progenitors are defined by specific surface marker expression, including CD34 and hematopoietin receptors.

Purpose of the Study:

  • To describe multiparametric flow cytometry panels for detecting and isolating human DC progenitors.
  • To provide a protocol for assessing in vitro differentiation and proliferation of DC progenitors.
  • To facilitate the study of human DC hematopoiesis in health, disease, and vaccine settings.

Main Methods:

  • Development of five multiparametric flow cytometry panels.
  • Optimization of antibody panels and gating strategies for immunostaining.
  • Application to bone marrow and cord blood specimens.

Main Results:

  • Simultaneous detection and phenotyping of four distinct DC progenitor populations.
  • Successful isolation of DC progenitors for in vitro studies.
  • Assessment of DC progenitor differentiation and proliferation capabilities.

Conclusions:

  • The described protocol enables rapid (3-7 h) and comprehensive analysis of human DC hematopoiesis.
  • This method is a valuable tool for immunological research, clinical diagnostics, and vaccine development.
  • Optimized flow cytometry panels and gating strategies enhance the study of DC progenitor biology.