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Model of Ischemia and Reperfusion Injury in Rabbits
Published on: November 3, 2023
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5-HT Receptor Antagonism Attenuates the Ischemia-Reperfusion Injury After Rabbit Lung Preservation
J L Arreola-Ramírez1, J Alquicira-Mireles1, P E Morales-Hernández1
1Departamento de Investigación en Hiperreactividad Bronquial, Instituto Nacional de Enfermedades Respiratorias, Mexico DF, Mexico.
Transplantation Proceedings
|August 22, 2015
Summary
Serotonin (5-HT) release increases during lung preservation, contributing to ischemia-reperfusion injury. Blocking serotonin receptors with methiothepin or SB204741 partially reduced lung vascular permeability after preservation.
Area of Science:
- Transplantation immunology
- Vascular physiology
- Pharmacology
Background:
- Lung transplantation success is limited by ischemia-reperfusion injury (IRI).
- IRI increases vascular permeability, a key pathological feature.
- The role of serotonin (5-hydroxytryptamine, 5-HT) in lung IRI is unknown.
Purpose of the Study:
- To measure 5-HT release during rabbit lung preservation.
- To evaluate the effect of 5-HT receptor antagonists on lung vascular permeability after preservation.
Main Methods:
- Rabbit lungs were preserved for 24 hours.
- 5-HT release was measured over time.
- Modified capillary filtration coefficient (mKf,c) was assessed.
- Effects of methiothepin and SB204741 on mKf,c were evaluated.
Main Results:
- 5-HT release peaked within 15 minutes of lung harvesting.
- 24-hour lung preservation significantly increased mKf,c.
- Methiothepin and SB204741 partially reduced the increase in mKf,c.
- SB204741 showed a greater reduction in mKf,c compared to methiothepin.
Conclusions:
- 5-HT is released during lung preservation.
- 5-HT contributes to increased vascular permeability in IRI.
- Targeting 5-HT receptors may mitigate lung IRI.
