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Human Virome

Human Virome

The human body harbors a vast and diverse viral community known as the human virome. The virome includes bacteriophages that infect bacteria, and eukaryotic viruses that infect human cells. Transient dietary and environmental viruses also contribute to this dynamic ecosystem. Estimates suggest the human body may contain on the order of 10¹³ viral particles, though abundance varies widely by body site and detection method.Comprehensive characterization of the virome has become possible...

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Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells

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Published on: November 12, 2015

Adenovirus 36 DNA in human adipose tissue.

E Ponterio¹, R Cangemi², S Mariani¹

¹Department of Experimental Medicine, Section of Medical Pathophysiology Food Science and Endocrinology, Sapienza University of Rome, Rome, Italy.

International Journal of Obesity (2005)

|August 22, 2015

Summary

Adenovirus 36 (Adv36) DNA was detected in the visceral adipose tissue of 19% of overweight or obese adults. This suggests a link between Adv36 infection and obesity, warranting further research.

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Area of Science:

Virology
Obesity Research
Molecular Biology

Background:

Emerging evidence suggests a potential link between adenovirus 36 (Adv36) and human obesity.
Data on adenoviral DNA within human adipose tissue remains scarce.

Purpose of the Study:

To investigate the presence of Adv36 DNA in the adipose tissue of overweight and obese adults.
To explore the potential role of Adv36 in human obesity.

Main Methods:

DNA extraction from adipose tissue biopsies of 21 adult patients.
Polymerase Chain Reaction (PCR) using primers specific to Adv36 fiber protein and E4orf1.
Sequence alignment with NCBI database and phylogenetic analysis using MEGA6 software.

Main Results:

Adv36 DNA was identified in 19% (4 out of 21) of the analyzed adipose tissue samples.
The findings confirm the presence of Adv36 in visceral adipose tissue of some individuals.

Conclusions:

This study provides evidence that Adv36 can be present in human visceral adipose tissue.
Further investigation is crucial to understand Adv36's effect on adipocytes, its prevalence, and its precise relationship with obesity.
These findings may inform the development of future vaccines against Adv36 infection.