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Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
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In Vitro Analyses of T Cell Effector Differentiation

Elizabeth A Wohlfert1, Andrea C Carpenter, Yasmine Belkaid

  • 1Department of Microbiology and Immunology, School of Medicine and Biomedical Sciences, University at Buffalo (SUNY), 251 Biomedical Research Building, Buffalo, NY, 14214, USA, wohlfert@buffalo.edu.

Methods in Molecular Biology (Clifton, N.J.)
|August 22, 2015
PubMed

In vitro culture is an important complement, or substitute, to in vivo approaches in order to study T cell effector differentiation. Here, we describe culture conditions that generate specific effector cell types by exposing naïve T cells to appropriate cytokine signals.

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