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NaHS Protects Cochlear Hair Cells from Gentamicin-Induced Ototoxicity by Inhibiting the Mitochondrial Apoptosis

Yaodong Dong1, Dongliang Liu1, Yue Hu1

  • 1Department of Otology, Shengjing Hospital Affiliated to China Medical University, Shenyang 110004, Liaoning, China.

Plos One
|August 22, 2015
PubMed

Insights

Sodium hydrosulfide (NaHS) protects against gentamicin-induced ototoxicity. This study shows NaHS preserves hair cell function and viability, offering potential for preventing aminoglycoside-induced hearing loss.

Area of Science:

  • Ototoxicity
  • Pharmacology
  • Cell Biology

Background:

  • Aminoglycoside antibiotics, like gentamicin, can cause ototoxicity by damaging cochlear sensory hair cells through oxidative stress and apoptosis.
  • The protective role of sodium hydrosulfide (NaHS) against aminoglycoside-induced hearing loss remains largely unexplored.

Purpose of the Study:

  • To investigate the antioxidant and anti-apoptotic effects of NaHS in protecting against gentamicin-induced ototoxicity in vitro.
  • To evaluate NaHS's potential as a therapeutic agent for preventing hearing loss caused by aminoglycoside antibiotics.

Main Methods:

  • Utilized in vitro cultured House Ear Institute-Organ of Corti 1 (HEI-OC1) cells and isolated mouse cochlea models.
  • Assessed cell viability, hair cell number, cochlear morphology, reactive oxygen species (ROS) production, mitochondrial depolarization, and apoptosis.
  • Measured mechanotransduction function in isolated cochlear explants.

Main Results:

  • NaHS demonstrated significant protective effects against gentamicin-induced ototoxicity in both HEI-OC1 cells and mouse cochlea.
  • NaHS treatment maintained cell viability, hair cell number, and cochlear morphology.
  • Reduced ROS production, mitochondrial depolarization, and apoptosis were observed, alongside preserved mechanotransduction in cochlear explants.

Conclusions:

  • NaHS exhibits potent antioxidant and anti-apoptotic properties that protect against gentamicin-induced ototoxicity.
  • NaHS preserves cochlear hair cell function and viability, indicating its potential as a therapeutic strategy for preventing aminoglycoside-induced hearing loss.

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