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Chromatin, DNA structure and alternative splicing.

Nicolás Nieto Moreno1, Luciana E Giono1, Adrián E Cambindo Botto1

  • 1Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-UBA-CONICET) and Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón 2, C1428EHA Buenos Aires, Argentina.

FEBS Letters
|August 23, 2015
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Summary

Template features regulate transcription and alternative splicing by controlling RNA polymerase II elongation. Non-B DNA conformations may also influence alternative splicing decisions, impacting gene expression.

Keywords:
Alternative splicingChromatin structureNon-B DNARNA polymerase II elongation

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Area of Science:

  • Molecular Biology
  • Genetics
  • Epigenetics

Background:

  • Transcription and alternative splicing are coupled processes.
  • Transcriptional elongation rate influences alternative splicing outcomes.
  • Template features like histone modifications and DNA-binding proteins can impede RNA polymerase II progression.

Purpose of the Study:

  • To review evidence linking template structural modifications to transcription and splicing.
  • To explore the potential role of non-B DNA conformations in alternative splicing regulation.

Main Methods:

  • Literature review and synthesis of existing research.
  • Discussion of mechanistic insights into transcription-splicing coupling.

Main Results:

  • Template roadblocks, including chromatin structure and proteins, affect elongation rate and splicing timing.
  • Non-B DNA structures are proposed as potential regulators of alternative splicing.

Conclusions:

  • Structural modifications of the DNA template significantly influence the interplay between transcription and splicing.
  • Non-B DNA conformations represent a novel area for investigating alternative splicing regulation.