Algimonas arctica sp. nov., isolated from intertidal sand, and emended description of the genus Algimonas

A novel Gram-reaction-negative, aerobic, pale-orange-pigmented bacterium, designated strain SM1216T, was isolated from Arctic intertidal sand. Cells of strain SM1216T were dimorphic rods with a single polar prostheca or flagellum. The strain grew at 4 − 30 °C (optimum at 25 °C) and with 0.5 − 6 % (w/v) NaCl (optimum with 2 − 3 %). It reduced nitrate to nitrite but did not hydrolyse gelatin, DNA or Tween 80. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain SM1216T was affiliated with the genus Algimonas in the family Hyphomonadaceae, sharing 97.5 and 96.3 % similarity with Algimonas ampicilliniresistens 14A-2-7T and Algimonas porphyrae 0C-2-2T, respectively, the two known species in the genus Algimonas. However, the level of DNA–DNA relatedness between strain SM1216T and the type strain of A. ampicilliniresistens, the nearest phylogenetic neighbour, was 57.9 %. The major cellular fatty acids of strain SM1216T were C18 : 1ω7c and C18 : 1 2-OH. The main polar lipids of strain SM1216T were monoglycosyldiglyceride (MGDG), glucuronopyranosyldiglyceride (GUDG), phosphatidylglycerol (PG) and three unidentified phospholipids (PL1–3). The major respiratory quinone was ubiquinone 10 (Q10). The genomic G+C content of strain SM1216T was 60.6 mol%. On the basis of the evidence from this polyphasic study, strain SM1216T represents a novel species in the genus Algimonas, for which the name Algimonas arctica sp. nov. is proposed. The type strain is SM1216T ( = MCCC 1K00233T = KCTC 32513T). An emended description of the genus Algimonas is also given.