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Updated: Apr 5, 2026

A Soluble Tetrazolium-Based Reduction Assay to Evaluate the Effect of Antibodies on Candida tropicalis Biofilms
Published on: September 16, 2022
Changes in the hemolytic activity of Candida species by common electrolytes
Lei Wan1,2, Gang Luo3, Haibin Lu4
1Department of Microbiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, China. wanlei1987@hotmail.com.
Background:
Hemolysins are crucial virulence factors which help pathogens to survive and persist in the host. This study investigated whether common electrolytes will affect the hemolysins of Candida species. The hemolysins from 25 Candida isolates were investigated using a plate specially designed for Candida species in the presence of three electrolytes, CaCl2, NaCl and KCl, at different concentrations. The hemolytic activity was determined after 48 h and the hemolytic index was calculated.
Results:
All three electrolytes caused a decrease in the hemolytic activity. Significant differences existed between varying concentrations of NaCl, while no significant differences existed for the CaCl2 and KCl groups. Additionally, the peripheral hemolytic index was highly correlated with the hemolytic index (r = 0.656, p < 0.001).
Conclusions:
Our findings indicate that electrolytes reduce hemolysis by Candida species and a correlation exists between the peripheral hemolytic index and the translucent hemolytic index.
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