Pseudoxanthomonas sangjuensis sp. nov., isolated from greenhouse soil

One bacterial strain, designated 5GH38-5(T), which was characterized as aerobic, Gram-staining-negative, non-flagellated rods, was isolated from a soil sample from a greenhouse in Sangju region, Republic of Korea. It grew at temperatures of 15-45 °C, pH 5.0-9.0 and NaCl concentrations (w/v) of 0-3.0%. 16S rRNA gene sequence analysis showed the strain was closely related to Pseudoxanthomonas kaohsiungensis J36(T) (97.3%), Pseudoxanthomonas suwonensis 4M1(T) (96.8%), Pseudoxanthomonas daejeonensis TR6-08(T) (96.7%) and Pseudoxanthomonas kalamensis JA40(T) (96.7%). Its major fatty acids were iso-C16 : 0, anteiso-C15 : 0 and iso-C15 : 0. The predominant ubiquinone was Q-8. The major polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content was 71.1 mol%. The DNA-DNA hybridization value between strain 5GH38-5(T) and P. kaohsiungensis J36(T) was less than 70%. The combined phenotypic, chemotaxonomic and phylogenetic data showed that strain 5GH38-5(T) could be clearly distinguished from closely related members of the genus Pseudoxanthomonas. Therefore, the results of this study indicated the existence of a novel species of the genus Pseudoxanthomonas, for which we propose the name Pseudoxanthomonas sangjuensis sp. nov., with strain 5GH38-5(T) ( = KACC 16961(T)= DSM 28345(T)= JCM 19948(T)) as the type strain.