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Updated: Apr 5, 2026

Visualization of ATP Synthase Dimers in Mitochondria by Electron Cryo-tomography
Published on: September 14, 2014
Assembly of human mitochondrial ATP synthase through two separate intermediates, F1-c-ring and b-e-g complex
Makoto Fujikawa1, Kanako Sugawara2, Tsutomu Tanabe3
1JST ICORP ATP-Synthesis Regulation Project, 2-3-6 Aomi, Koto-ku, Tokyo 135-0064, Japan; Dep. of Phamacol. and Neurobiol., Grad. School of Med., Tokyo Medical and Dental Univ., 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8519, Japan.
Abstract:
Mitochondrial ATP synthase is a motor enzyme in which a central shaft rotates in the stator casings fixed with the peripheral stator stalk. When expression of d-subunit, a stator stalk component, was knocked-down, human cells could not form ATP synthase holocomplex and instead accumulated two subcomplexes, one containing a central rotor shaft plus catalytic subunits (F1-c-ring) and the other containing stator stalk components ("b-e-g" complex). F1-c-ring was also formed when expression of mitochondrial DNA-coded a-subunit and A6L was suppressed. Thus, the central rotor shaft and the stator stalk are formed separately and they assemble later. Similar assembly strategy has been known for ATP synthase of yeast and Escherichia coli and could be common to all organisms.
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