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Effect of agitation rate and impeller design on oxygen transfer coefficients in small bioreactors using surface
E M Sybert1, E L Winter, T W Cadman
1Engineering Research Center, University of Maryland, College Park 20742.
Biotechniques
|April 1, 1989
Abstract:
The effects of agitation rate and impeller type on the combined oxygen mass-transfer coefficient (kL a) in four different benchtop bioreactors have been examined. Surface oxygenation of a cell culture medium supplemented with fetal bovine serum and distilled deionized water has been studied by passing air through the bioreactor headspace at approximately one headspace volume per minute. A new ribbon-type impeller design using strips of Teflon has been shown to be superior to conventional impeller designs for oxygen transfer.