[Pro-and anti-nucleation activity of biliary proteins in gallbladder bile with and without gallstones]

The proteins of pro-and anti-nucleation activity in gallbladder bile were isolated by chromatography on Bio Gel P-100 and Con A Sepharose. The authors for the first time showed the evidence of presence of pro-and anti-nucleation activity proteins co-existing in gallbladder bile of patients with and without cholesterol gallstones. The result of further study showed that Transferrin, one of the glycoproteins in gallbladder bile, had the effect of promoting nucleation activity on rapid formation of cholesterol crystal in artificial supersaturated bile. The difference in pro-and anti-nucleation proteins of gallbladder bile between patients with and those without cholesterol gallstones were also shown in this paper.