Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The Lipotubuloids of <i>Ornithogalum umbellatum</i> L. Contain Hyperstable Microtubules.

Plants (Basel, Switzerland)·2025

Same author

Structural Conservation of Phi Thickenings in Diverse Plant Taxa.

Annals of botany·2025

Same author

The <i>Arabidopsis thaliana</i> Double-Stranded RNA Binding Proteins DRB1 and DRB2 Are Required for miR160-Mediated Responses to Exogenous Auxin.

Genes·2025

Same author

Molecular Manipulation of the miR160/<i>AUXIN RESPONSE FACTOR</i> Expression Module Impacts Root Development in <i>Arabidopsis thaliana</i>.

Genes·2024

Same author

Characterisation of the trans-membrane nucleoporins GP210 and NDC1 in Arabidopsis thaliana.

Plant science : an international journal of experimental plant biology·2023

Same author

Asymmetric wall ingrowth deposition in Arabidopsis phloem parenchyma transfer cells is tightly associated with sieve elements.

Journal of experimental botany·2022

Same journal

PlasmiDB: an open-source and customizable database for plasmid lifecycle management in multi-user, multi-project plant molecular biology laboratories.

Plant methods·2026

Same journal

Establishment of a protoplast isolation and transient transformation system for tung tree (Vernicia fordii).

Plant methods·2026

Same journal

Deep aerenchyma: a transformer-based pipeline for scalable phenotyping of rice root aerenchyma lacunae across environments.

Plant methods·2026

Same journal

Comparative analysis of SP3 and S-Trap sample preparation protocols for proteomic profiling associated with somatic embryogenesis efficiency in Olea europaea L.

Plant methods·2026

Same journal

DAPR-AM-Net: an end-to-end smart farming system powered by dual-attention progressive refinement and adaptive MixUp for explainable tomato leaf disease classification and forecasting.

Plant methods·2026

Same journal

Deep soil layers show the most pronounced genetic variation in wheat root length.

Plant methods·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 5, 2026

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection

Published on: February 24, 2026

Optimisation approaches for concurrent transmitted light imaging during confocal microscopy.

David A Collings¹

¹Biomolecular Interaction Centre, School of Biological Sciences, University of Canterbury, Private Bag 4800, Christchurch, 8140 New Zealand.

|August 25, 2015

Summary

This summary is machine-generated.

Transmitted light detectors on confocal microscopes offer valuable cellular context for plant cell imaging. This study presents methods to improve transmitted light images, enabling quantification of pigment changes in plant cells.

Keywords:

Anthocyanin Confocal microscopy Green fluorescent protein Köhler illumination Plant pigments Quantification Real colour imaging Transmitted light detector

More Related Videos

High-plex Imaging using Spectral Confocal Microscopy to Minimize Non-specific Tissue Fluorescence

High-plex Imaging using Spectral Confocal Microscopy to Minimize Non-specific Tissue Fluorescence

Published on: October 28, 2025

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Related Experiment Videos

Last Updated: Apr 5, 2026

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection

Published on: February 24, 2026

High-plex Imaging using Spectral Confocal Microscopy to Minimize Non-specific Tissue Fluorescence

High-plex Imaging using Spectral Confocal Microscopy to Minimize Non-specific Tissue Fluorescence

Published on: October 28, 2025

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Area of Science:

Plant cell biology
Microscopy techniques

Background:

Confocal microscopy's transmitted light detectors are underutilized for live plant cell imaging.
These detectors provide cellular context and are spatially/temporally registered with fluorescence images.

Purpose of the Study:

To document approaches for improving transmitted light images in plants.
To enable quantification of pigment changes in plant cells using transmitted light imaging.

Main Methods:

Köhler illumination for correct light path alignment.
Sequential scanning with RGB lasers for true color imaging of pigmented samples.
Image optimization and merging in ImageJ.
Using non-absorbed wavelengths for faster imaging.
Employing colored glass filters to enhance images of fluorescently tagged samples.

Main Results:

Optimized transmitted light images maintain registration with fluorescence images.
Color changes in plant pigments (e.g., anthocyanins) can be quantified.
Demonstrated improved imaging of Arabidopsis leaves expressing GFP by blocking blue light.

Conclusions:

Transmitted light detectors are effective for documenting and measuring pigment changes in plant cells.
These images provide crucial context for confocal microscopy data.