A Versatile Monoclonal Antibody Specific Against Human DAB2IP.

Area of Science:

• Oncology
• Molecular Biology
• Immunology

Background:

• Human DAB2 interaction protein (DAB2IP) is a Ras-GTPase activating protein family member.
• DAB2IP functions as a tumor suppressor, suggesting its potential as a cancer prognostic biomarker.

Purpose of the Study:

• To generate a specific monoclonal antibody against human DAB2IP.
• To validate the antibody as a tool for DAB2IP research and biomarker development.

Main Methods:

• Generation of a mouse monoclonal antibody (2A4) against human DAB2IP.
• Characterization of the antibody's specificity for both native and denatured DAB2IP.
• Isotyping the antibody as IgG1.

Main Results:

• A specific monoclonal antibody, 2A4, was successfully generated against human DAB2IP.
• The antibody recognizes human DAB2IP in both native and denatured states.
• The antibody was confirmed to be of the IgG1 isotype.

Conclusions:

• The 2A4 antibody is a specific and versatile tool for studying human DAB2IP.
• This antibody will facilitate further research into the mechanisms of DAB2IP.
• The antibody supports the investigation of DAB2IP's prognostic significance in cancers.