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Para-toluenesulfonamide induces tongue squamous cell carcinoma cell death through disturbing lysosomal stability

Zhe Liu1, Chenyuan Liang, Zhuoyuan Zhang

  • 1aDepartment of Head and Neck Oncology bState Key Laboratory of Oral Diseases cDepartment of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu dState Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Diseases, First Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.

Anti-Cancer Drugs
|August 25, 2015
PubMed

Insights

Para-toluenesulfonamide (PTS) effectively inhibits tongue squamous cell carcinoma (Tca-8113) by inducing cell death through lysosomal and mitochondrial damage. This study reveals PTS

Area of Science:

  • Oncology
  • Cell Biology
  • Pharmacology

Background:

  • Para-toluenesulfonamide (PTS) exhibits potential anticancer properties against various tumors.
  • Investigating PTS' specific effects on tongue squamous cell carcinoma (Tca-8113) is crucial for targeted therapy development.

Purpose of the Study:

  • To evaluate the inhibitory effects of PTS on Tca-8113 cells.
  • To elucidate the mechanisms of PTS-induced cell death, focusing on lysosomal and mitochondrial pathways.
  • To assess PTS selectivity for cancer cells over normal fibroblasts.

Main Methods:

  • High-performance liquid chromatography (HPLC) for PTS accumulation analysis.
  • Cell viability, invasion, and cell death assays (flow cytometry, lactate dehydrogenase release).
  • Morphological analysis, lysosomal integrity assays, and Western blotting for cathepsin B and cytochrome c.
  • ATP biosynthesis and mitochondrial function assessment.

Main Results:

  • PTS selectively accumulated in Tca-8113 cells.
  • PTS significantly reduced cell viability and invasion while increasing cell death.
  • PTS induced simultaneous apoptosis and necrosis, disrupting lysosomal stability and activating cathepsin B.
  • PTS inhibited ATP production and triggered mitochondrial cytochrome c release.

Conclusions:

  • PTS demonstrates potent inhibitory effects on tongue squamous cell carcinoma.
  • PTS induces cancer cell death via lysosomal and mitochondrial pathways.
  • PTS shows potential as a therapeutic agent for tongue cancer.

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