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Related Concept Videos

Catenins01:23

Catenins

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Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the...
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Cadherins in Tissue Organization01:19

Cadherins in Tissue Organization

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The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...
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Structure of Cadherins01:25

Structure of Cadherins

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The cadherins were one of the first cell adhesion molecules discovered; the term “cadherins”   is based on their calcium-dependent adhering properties. The first cadherins discovered on the epithelial, neuronal, and placental cells were named E-cadherin, P-cadherin, and N-cadherin, respectively. These classical cadherins share sequence and structural similarities. Other cadherins, including those involved in cell signaling, are grouped into non-classical cadherins. This...
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Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

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Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
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Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

9.9K
The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
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Anchoring Junctions01:03

Anchoring Junctions

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Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...
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Related Experiment Video

Updated: Apr 5, 2026

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules
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Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules

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Distinct E-cadherin-based complexes regulate cell behaviour through miRNA processing or Src and p120 catenin

Antonis Kourtidis1, Siu P Ngok1, Pamela Pulimeno2

  • 1Department of Cancer Biology, Mayo Clinic Comprehensive Cancer Center, Mayo Clinic, 4500 San Pablo Road, Jacksonville, Florida 32224, USA.

Nature Cell Biology
|August 25, 2015
PubMed
Summary
This summary is machine-generated.

Epithelial cells have distinct junctional complexes that control cell growth. A complex involving PLEKHA7 and the microprocessor suppresses tumor-promoting genes, maintaining cell homeostasis.

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Related Experiment Videos

Last Updated: Apr 5, 2026

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Cancer Research

Background:

  • E-cadherin and p120 catenin are crucial for epithelial homeostasis but can promote tumor growth.
  • The dual role of cell adhesion molecules in cancer requires further investigation.

Purpose of the Study:

  • To elucidate the distinct functions of junctional complexes in regulating cell behavior.
  • To identify the molecular mechanisms underlying the paradoxical roles of E-cadherin and p120 catenin.

Main Methods:

  • Co-immunoprecipitation assays to identify protein interactions.
  • Analysis of microRNA processing and its impact on gene expression.
  • Spatial localization studies of junctional proteins and microprocessor components.

Main Results:

  • Two distinct junctional complexes were identified: a growth-suppressing apical complex and a growth-promoting basolateral complex.
  • PLEKHA7 mediates the recruitment of DROSHA and DGCR8 to the apical zonula adherens.
  • The PLEKHA7-microprocessor complex processes pri-miRNAs, suppressing tumor-promoting genes like SNAI1, MYC, and CCND1.

Conclusions:

  • Adhesion complexes can regulate cellular behavior through association with the microprocessor complex.
  • PLEKHA7-mediated microRNA processing at the apical junction suppresses pro-tumorigenic pathways.