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Detection of DNA double-strand breaks in boron neutron capture reaction
Emiko Okamoto1, Tetsuya Yamamoto2, Kei Nakai2
1Department of Neurosurgery, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan; Doctoral Program in Clinical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan.
Abstract:
We evaluated DNA double-strand breaks (DSBs) induced by boron neutron capture reaction (BNCR) using plasmid DNA, boron solution, and gel electrophoresis. The amount of the linear form of DNA produced by DSBs increased with the neutron-beam irradiation dose. The amount of the open-circular form of DNA produced by single-strand breaks (SSBs) increased with the neutron-beam irradiation dose and the (10)B concentration. The model facilitated quantification of BNCR-induced DSBs and SSBs, irrespective of the DNA repair mechanism.
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